Molecular, cellular and phylogenetic aspects of selective inhibition of early periods in metazoan ontogenesis by sponge and other substances

2013 Fiscal Year Final Research Report

• Project/Area Number: 23658102
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Keio University
• Principal Investigator: IKEGAMI Susumu 慶應義塾大学, 自然科学研究教育センター, 訪問教授 (80011980)
• Co-Investigator(Kenkyū-buntansha): KANEKO Hiroyuki 慶應義塾大学, 文学部, 教授 (20169577)
• Project Period (FY): 2011 – 2013
• Keywords: ヒトデ発生 / 発生阻害

Research Abstract

Following fertilization, the starfish, Asterina pectinifera, embryo develops to the blastula and then undergoes gastrulation to form a gut and create a feeding bipinnaria larva. The larva then takes on a more elaborate form called a brachioraria larva. Several substances obtained from sponges specifically prevent starfish embryonic development at the blastula or the gastrula stages. Screening search for synthetic compounds which mimic the action of these substances was undertaken but such compounds were not detected.
The study was then focused on the effect of loss of nuclear transglutaminase, a protein that is phylogenetically unique in the starfish embryo, by microinjecting the morpholino antisense oligonucleotide. The injected embryo developed normally through the embryonic and bipinnaria stages but was unable to form normal brachioraria. This result suggests that the unique protein is involved in the morphogenetic event at the latter period in the starfish ontogeny.

Research Products

• [Journal Article] Petroacety- lene, a new polyacetylene from the marine sponge Petrosia solida that inhibits blastulation of starfish embryos (2013)
  • Author(s): Ohta, S., Ogawa, T., Ohta, E., Ikeuchi, T., Kamemura, K. , & Ikegami, S
  • Journal Title: Natural Product Research Volume: Vol. 27 Pages: 1842-1847
  • DOI: 10.1080/14786419.2012.763128
  • Peer Reviewed
• [Journal Article] Bromotheoynic acid, a brominated acetylenic acid from the marine sponge Theonella swinhoei (2013)
  • Author(s): Aoki, N., Yamamoto, K., Ogawa, T., Ohta, E., Ikeuchi, T., Kamemura, K., Ikegami, S., & Ohta, S
  • Journal Title: Natural Products Research Volume: Vol. 27 Pages: 117-122
  • DOI: 10.1080/14786419.2012.660636
  • Peer Reviewed
• [Journal Article] Starfish ApDOCK protein essentially functions in larval defense system operated by mesenchyme cells (2012)
  • Author(s): Furukawa, R., Funabashi, H., Matsumoto, M., & Kaneko, H
  • Journal Title: Immunol. Cell Biol Volume: Vol. 90 Pages: 995-965
  • DOI: 10.1038/icb.2012.37
  • Peer Reviewed
• [Journal Article] Characterization of O-GlcNAcylation in starfish (Asterina pectinifera) development from fertilization to bipinnaria larva (2011)
  • Author(s): Ogawa, M., Adachi, T., Ikegami, S., Kato, K. H., Yamamoto, A., & Kamemura, K
  • Journal Title: Biosci. Biotechnol. Biochem Volume: Vol. 75 Pages: 358-361
  • DOI: 10.1271/bbb.100601
  • Peer Reviewed
• [Journal Article] Uneven distribution pattern and increasing numbers of mesenchyme cells during development in the starfish, Asterina pectinifera (2011)
  • Author(s): Hamanaka, G., Hosaka, E., Kuraishi, R., Hosoya, N., Matsumoto, M., & Kaneko, H
  • Journal Title: Dev. Growth Differ Volume: Vol. 53 Pages: 440-449
  • DOI: 10.1111/j.1440-169X.2011.01259.x
  • Peer Reviewed
• [Presentation] イトマキヒトデ胚発生における核型トランスグルタミナーゼの機能について (2013)
  • Author(s): 池上 晋, 古川亮平,金子洋之
  • Organizer: 日本動物学会大会第84回大会
  • Place of Presentation: 岡山大学津島地区一般教育棟
  • Year and Date: 2013-09-28
• [Presentation] イトマキヒトデ胚発生における間充織の分化 (2012)
  • Author(s): 池上 晋, 清水孝彦, 金子洋之
  • Organizer: 日本動物学会大会第83回大会
  • Place of Presentation: 大阪大学豊中キャンパス全学教育推進管理・講義棟
  • Year and Date: 2012-09-13