2013 Fiscal Year Final Research Report
Establishment of a simple, easy and useful new recombinant virus manufacture system using a homologous recombination phenomenon
| Project/Area Number |
23658240
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Kyoto University |
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Principal Investigator |
MIURA Tomoyuki 京都大学, ウイルス研究所, 准教授 (40202337)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 感染症 / 組換えウイルス / エイズ / SHIV / HIV-1mt / デングウイルス / ダニ媒介性脳炎ウイルス |
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| Research Abstract |
I examined the influence that the length of the overlap domain, a position, and homology give in homologous recombination efficiency, and also the conditions such as the gene introduction method and the gene introduction cells using the conventional and new generation SHIV, and clarified the optimum. By this way, I succeeded in rearranging the env region of the various kinds of CCR5 type HIV-1 clinical isolates with maintaining variety in conventional and new generation SHIV as a backbone. Furthermore, I succeeded in an application of the method to Dengue virus and Tick-bone encephalitis virus construction.
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[Journal Article] Generation of a replication-competent chimeric simian-human immunodeficiency virus carrying env from subtype C clinical isolate through intracellular homologous recombination2013
Author(s)
Fujita, Y., Otsuki, H., Watanabe, Y., Yasui, M., Kobayashi, T., Miura, T., and Igarashi, T
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Journal Title
Virology
Volume: 436
Pages: 100-111
Peer Reviewed
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[Journal Article] Generation of a replication-competent simianhuman immunodeficiency virus, the neutralisation sensitivity of which can be enhanced in the presence of a small molecule CD4 mimic
Author(s)
Otsuki, H., Hishiki, T., Miura, T., Hashimoto, C., Narumi, T., Tamamura, H., Yoshimura, K., Matsushita, S., and Igarashi, T
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Journal Title
J. Gen. Virol
Volume: 94
Pages: 2710-2716
Peer Reviewed
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[Presentation] CCR5 指向性を示す新規サル指向性HIV-1 はサル個体に持続感染する2013
Author(s)
齊藤暁, 大附寛幸, 東濃篤徳, 鈴木紗織, 松田健太, 高橋尚史, 岩谷靖雅, 杉浦亙, 野間口雅子, 足立昭夫, 保富康宏, 俣野哲朗, 三浦智行, 明里宏文
Organizer
第27回日本エイズ学会学術集会
Place of Presentation
熊本
Year and Date
20131120-22
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[Presentation] CCR5 指向性を示す新規サル指向性HIV-1 はサル個体に持続感染する2013
Author(s)
齊藤暁, 大附寛幸, 東濃篤徳, 鈴木紗織, 松田健太, 高橋尚史, 岩谷靖雅, 杉浦亙, 野間口雅子, 足立昭夫, 保富康宏, 俣野哲朗, 三浦智行, 明里宏文
Organizer
第61回日本ウイルス学会学術集会
Place of Presentation
神戸
Year and Date
20131110-12
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[Presentation] ウイルスの標的細胞指向性はサル指向性HIV-1 の増殖に影響するか?2013
Author(s)
齊藤暁, 大附寛幸, 東濃篤徳, 鈴木紗織, 松田健太, 高橋尚史, 岩谷靖雅, 杉浦亙, 保富康宏, 俣野哲朗, 三浦智行, 明里宏文
Organizer
第156回日本獣医学会学術集会
Place of Presentation
岐阜
Year and Date
20130920-22
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