2013 Fiscal Year Final Research Report
Development of a new miRNA delivery system for targeting cancer stem cells
| Project/Area Number |
23659626
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
UEDA Shinobu 東京医科大学, 医学部, 助手 (00521874)
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| Co-Investigator(Kenkyū-buntansha) |
KURODA Masahiko 東京医科大学, 医学部, 教授 (80251304)
TAKANASHI Masakatsu 東京医科大学, 医学部, 講師 (80312007)
OHNO Shinichiro 東京医科大学, 医学部, 助教 (90513680)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUCHIDA Akihiko 東京医科大学, 医学部, 教授 (50207396)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 乳がん / miRNA / エキソソーム |
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| Research Abstract |
It is known to dysregulation of microRNAs contribution to the development and progression of cancers. MiRNAs in serum correlate with the cancer, cardiovascular disorders and the various types of diseases. In addition, extracellular miRNAs have been detected in exosomes isolated from numerous body fluids including serum. In this study, we examined whether exosomes were a new useful tools for gene delivery in a model of cancer. Modified exosomes with the GE11 peptide that binds to EGFR including let-7a were prepared (let-7a/GE11 exosomes). let-7a/GE11 exosomes were intravenously injected into Rag2-/- mice transplanted breast cancer cells (HCC-70). After four injections, let-7a/GE11 exosomes markedly suppressed tumor growth. These results suggest that exosomes may provide an effective miRNA delivery system for cancer therapy.
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[Journal Article] GKR6-deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance2013
Author(s)
Nakaya M, Tajima M, Kosako H, Nakaya T, Hashimoto A, Watari K, Nishihara H, Ohba M, Komiya S, Tani N, Nishida M, Taniguchi H, Sato Y, Matsumoto M, Tsuda M, Kuroda M, Inoue K, and Kurose H
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Journal Title
Nature Communications
Volume: 4
Pages: 1532
DOI
Peer Reviewed
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[Journal Article] The expression level of miR-18b in hepatocellular carcinoma is associated with the grade of malignancy and prognosis2013
Author(s)
Murakami Y, Tamori A, Itami S, Tanahashi T, Toyoda H, Tanaka M, Wu W, Brojigin N, Kaneoka Y, Maeda A, Kumada T, Kawada N, Kubo S and Kuroda M
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Journal Title
BMC Cancer.(Section : Genetics, genomics and epigenetics)
Volume: 13
Pages: 99
DOI
Peer Reviewed
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[Journal Article] Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells2013
Author(s)
Ohno S, Takanashi M, Sudo K, Ueda S, Ishikawa A, Matsuyama N, Fujita K, Mizutani T, Ohgi T, Ochiya T, Gotoh N, Kuroda M
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Journal Title
Molecular Therapy
Volume: 21(1)
Pages: 185-191
DOI
Peer Reviewed
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[Journal Article] TLS-CHOP represses miR-486 expression, inducing upregulation of a metastasis regulator PAI-1 in human myxoid liposarcoma2012
Author(s)
Borjigin N, Ohno S, Wu W, Tanaka M, Suzuki R, Fujita K, Takanashi M, Oikawa K, Goto T, Motoi T, Kosaka T, Yamamoto K, Kuroda M
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 427(2)
Pages: 355-60
DOI
Peer Reviewed
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[Journal Article] Drug-resistant colon cancer stem cells are Lgr5-negative and are capable of tumor reconstitution progressing through a Lgr5-positive state2012
Author(s)
Kobayashi S, Yamada-Okabe H, Suzuki M, Natori O, Kato A, Matsubara K, Chen YJ, Yamazaki M, Funahashi S, Yoshida K, Hashimoto E, Watanabe Y, Mutoh H, Ashihara M, Kato C, Watanabe T, Yoshikubo T, Tamaoki N, Ochiya T, Kuroda M, Levine AJ, and Yamazaki T
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Journal Title
Stem cell
Volume: 30(12)
Pages: 2631-44
DOI
Peer Reviewed
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[Journal Article] Efficacy of a Novel Class of RNA Interference Therapeutic Agents2012
Author(s)
Hamasaki T, Suzuki H, Shirohzu H, Matsumoto T, D'Alessandro-Gabazza CN, Gil-Bernabe P, Boveda-Ruiz D, Naito M, Kobayashi T, Toda M, Mizutani T, Taguchi O, Morser J, Eguchi Y, Kuroda M, Ochiya T, Hayashi H, Gabazza EC, Ohgi T
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Journal Title
PLoS One
Volume: 7(8)
Pages: e42655
DOI
Peer Reviewed
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[Journal Article] Regulation of the development of acute hepatitis by IL-23 through IL-22 and IL-17 production2011
Author(s)
Xu M, Morishima N, Mizoguchi I, Chiba Y, Fujita K, Kuroda M, Iwakura Y, Cua DJ, Yasutomo K, Mizuguchi J, Yoshimoto T
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Journal Title
Eur J Immunol
Volume: 41(10)
Pages: 2828-39
DOI
Peer Reviewed
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[Journal Article] Dysregulation of IFN system can lead to poor response to pegylated interferon and ribavirin therapy in chronic hepatitis C2011
Author(s)
Onomoto K, Morimoto S, Kawaguchi T, Toyoda H, Tanaka M, Kuroda M, Uno K, Kumada T, Matsuda F, Shimotohno K, Fujita T, Murakami Y
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Journal Title
PLoS One
Volume: 6(5)
Pages: e19799
DOI
Peer Reviewed
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