Potential of microRNA as a biomarker for atherosclerotic aortic aneurysm

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659670
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Thoracic surgery
• Research Institution: Osaka University
• Principal Investigator: TORIKAI Kei 大阪大学, 医学系研究科, 助教 (70364792)
• Co-Investigator(Kenkyū-buntansha): SAWA Yosiki 大阪大学, 大学院・医学系研究科, 教授 (00243220) ; KURATANI Toru 大阪大学, 大学院・医学系研究科, 寄附講座 教授 (90448035) ; SAKAGUCHI Taichi 大阪大学, 大学院・医学系研究科, 寄附講座 教授 (10467574) ; SHIRAKAWA Yukitoshi 大阪大学, 大学院・医学系研究科, 寄附講座 教授 (20457013) ; SHIMAMURA Kazuo 大阪大学, 医学部附属病院, 医員 (10507205) ; KIN Keiwa 大阪大学, 大学院・医学系研究科, 寄附講座助教 (70532985)
• Project Period (FY): 2011 – 2012
• Keywords: miRNA / 大動脈瘤 / AAA

Research Abstract

This study examined tissue- and plasma-microRNAs specifically associated with AAA. AAA and normal wall tissues were sampled from patients undergoing AAA repair and aortic valve replacement surgery, respectively. MicroRNA expression was assessed by high-throughput microRNA arrays and validated by real-time PCR for individual microRNAs that showed significant expression differences in the initial screening. MicroRNAs related to fibrosis (miR-29b), inflammation (miR-124a, miR-146a, miR-155 and miR-223), and endothelium (miR-126, let-7 family members and miR-21) were significantly upregulated in AAA tissue. Expression levels of monocyte chemoattractant protein-1 and miR-124a, 146a and 223, tumor necrosis factor-α and miR-126 and 223, and transforming growth factor-β and miR-146a showed a significant negative correlation. Expression of microRNAs, such as miR-29b, miR-124a, miR-155 and miR-223, upregulated in AAA tissue was significantly reduced in plasma of AAA patients compared to healthy controls and coronary artery disease patients.

Research Products

• [Journal Article] Tissue- and plasma-specific microRNA signatures for atherosclerotic abdominal aortic aneurysm (2012)
  • Author(s): Kin K, Miyagawa S, Fukushima S, Shirakawa Y, Torikai, K, Shimamura K, Daimin T, Kawahara Y, Kuratani T, Sawa Y
  • Journal Title: Journal of the American Heart Association Volume: 1 Pages: e000745
  • DOI: doi:10.1161/JAHA.112.000745
  • Peer Reviewed
• [Presentation] Potential of microRNA as a biomarker for atherosclerotic aortic aneurysm (2012)
  • Author(s): Keiwa Kin
  • Organizer: 第76回日本循環器学会総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 20120316-18
• [Presentation] Tissue- and plasma-specific microRNA signatures for atherosclerotic abdominal aortic aneurysm (2011)
  • Author(s): Keiwa Kin
  • Organizer: American Heart Association 2011
  • Place of Presentation: Orlando USA
  • Year and Date: 20111112-16
• [Presentation] Comprehensive analysis of microRNA expression in human abdominal aortic aneurysm (2011)
  • Author(s): Keiwa Kin
  • Organizer: 第75回日本循環器学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20110803-04