Determination of cell type(s) that should be transplanted into lesion core of the brain for better outcome

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659688
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: Ehime University
• Principal Investigator: TANAKA Junya 愛媛大学, プロテオ医学研究センター, 教授 (70217040)
• Co-Investigator(Kenkyū-buntansha): YANO Hajime 愛媛大学, 大学院・医学系研究科, 准教授 (00284414) ; TAKAHASHI Hisaaki 愛媛大学, 医学部附属病院, 講師 (20363228) ; SUGIMOTO Kana 愛媛大学, 大学院・医学系研究科, 助教 (00581034)
• Co-Investigator(Renkei-kenkyūsha): OHNISHI Takanori 愛媛大学, 大学院・医学系研究科, 教授 (70233210) ; KUMON Yoshiaki 愛媛大学, 大学院・医学系研究科, 准教授 (80127894)
• Project Period (FY): 2011 – 2012
• Keywords: 脳梗塞 / マイクログリア / 脳損傷 / アストロサイト / 細胞移植 / マクロファージ / 骨髄 / BINCs

Research Abstract

To determine cell type(s) that should be transplanted into the lesion core of ischemic and traumatic brain injuries with an aim to ameliorate the outcomes, several kinds of cultured neural cells including microglial cells, astrocytes, and brain macrophages isolated from ischemic lesion core of rat brains. Consequently,it was found that most of transplanted cells were eliminated by phagocytic macrophages in spite of the isotransplantation. Only BINCs were transplantable into the ischemic core, and their transplantation may be appeared therapeutically effective.

Research Products

• [Journal Article] Expression of MCP-1 and fractalkine on endothelial cells and astrocytes may contribute to the invasion and migration of brain macrophages in ischemic rat brain lesions (2013)
  • Author(s): Nari Tei, Junya Tanaka, Kana Sugimoto, Tasuku Nishihara, Ryutaro Nishioka, Hisaaki Takahashi, Hajime Yano, Shirabe Matsumoto, Shiro Ohue, Hideaki Watanabe, Yoshiaki Kumon, Takanori Ohnishi
  • Journal Title: J Neurosci Res Volume: 91 Pages: 681-693
  • Peer Reviewed
• [Journal Article] Anticonvulsive effect of paeoniflorin on experimental febrile seizures in immature rats: possible application for febrile seizures in children (2012)
  • Author(s): Hitomi Hino, Hisaaki Takahashi, Yuka Suzuki, Junya Tanaka, Eiichi Ishii, Mitsumasa Fukuda
  • Journal Title: PLoS ONE Volume: 7 Pages: e42920
  • DOI: DOI:10.1371/journal.pone.0042920
  • Peer Reviewed
• [Journal Article] Zonisamide up-regulated them RNAs encoding astrocytic anti-oxidative and neurotrophic factors. (2012)
  • Author(s): Choudhury ME, Sugimoto K, Kubo M, Iwaki H, Tsujii T, Kyaw WT, Nishikawa N, Nagai M, Tanaka J, Nomoto M
  • Journal Title: Eur J Pharmacol Volume: 689 Pages: 72-80
  • Peer Reviewed
• [Journal Article] Premetastatic vasculogenesis in oral squamous cell carcinoma xenograft-draining lymph nodes (2012)
  • Author(s): Mayorca-Guiliani AE, Yano H, Nakashiro KI, Hamakawa H, Tanaka J
  • Journal Title: Oral Oncology Volume: 48 Pages: 663-670
  • Peer Reviewed
• [Journal Article] Oct-3/4 promotes migration and invasion of glioblastoma cells (2012)
  • Author(s): Kobayashi K, Takahashi H, Inoue A, Harada H, Toshimori S, Kobayashi Y, Goto K, Sugimoto K, Yano H, Ohnishi T, Tanaka J
  • Journal Title: J Cell Biochem Volume: 113 Pages: 508-517
  • Peer Reviewed
• [Journal Article] Transient ischemia-induced paresis and complete paraplegia displayed distinct reactions of microglia and macrophages (2011)
  • Author(s): NakataT, Kawachi K, Nagashima M, Yasugi T, Izutani H, Ryugo M, Okamura T, Shikata F, Imagawa H, Yano H, Takahashi H, Tanaka J
  • Journal Title: Brain Res Volume: 1420 Pages: 114-124
  • Peer Reviewed
• [Journal Article] A cytokine mixture of GM‐CSF and IL‐3 that induces a neuroprotective phenotype of microglia leading to amelioration of (6‐OHDA)‐induced Parkinsonism of rats (2011)
  • Author(s): Choudhury ME, Sugimoto K, Kubo M, Nagai M, Nomoto M, Takahashi H, Yano H, Tanaka J
  • Journal Title: Brain Behav Volume: 1 Pages: 26-43
  • Peer Reviewed
• [Journal Article] Subcutaneous injection containing IL-3 and GM-CSF ameliorates stab wound-induced brain injury in rats (2011)
  • Author(s): Nishihara, Michihisa Ochi, Kana Sugimoto, Hisaaki Takahashi, Hajime Yano, Yoshiaki Kumon, Takanori Ohnishi, Junya Tanaka
  • Journal Title: Exp Neurol Volume: 229 Pages: 507-516
  • Peer Reviewed
• [Presentation] Agents modulating neuroprotective and neurotoxic functions of microglia and their application to the pathologic brains (2013)
  • Author(s): Junya Tanaka
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京都
  • Year and Date: 20130327-29
• [Presentation] Response of glial cells in and around ischemic lesion of rats that were subjected to transient middle cerebral artery occlusion: the involvement of IL-18 (2013)
  • Author(s): Ayano Mise, Kana Sugimoto, Ryutaro Nishioka, Hisaaki Takahashi, Hajime Yano, Junya Tanaka
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京都
  • Year and Date: 20130327-29
• [Presentation] Invasion of monocytes/macrophages into ischemic lesion of rat brain: involvement of chemokines and their receptors (2013)
  • Author(s): Yudai Ohara, Kana Sugimoto, Ryutaro Nishioka, Hisaaki Takahashi, Hajime Yano、Nari Tei, Yoshiaki Kumon, Takanori Ohnishi, Junya Tanaka
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京都
  • Year and Date: 20130327-29
• [Presentation] A cytokine mixture containing GM-CSF and IL-3 targeting microglia ameliorates neurological deficits of Parkinson's disease model rats (2012)
  • Author(s): Junya Tanaka, Mohammed E. Choudhury, Kana Sugimoto, Hisaaki Takahashi, Hajime Yano
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 大分県由布市
  • Year and Date: 20120803-04
• [Presentation] 脳梗塞巣に集積するマクロファージの役割とその治療的利用 (2012)
  • Author(s): 田中潤也、杉本香奈、西原佑、高橋寿明、矢野元
  • Organizer: 第22回日本病態生理学会
  • Place of Presentation: 大分県由布市
  • Year and Date: 20120803-04
• [Remarks]
  • URL: http://www.m.ehime-u.ac.jp/school/kisogp/contents/laboratories
• [Patent(Industrial Property Rights)] 免疫細胞の活性化抑制剤およびその用途 (2012)
  • Inventor(s): 田中潤也
  • Industrial Property Rights Holder: 国立大学法人愛媛大学
  • Industrial Property Number: 特願2012-173405
  • Filing Date: 2012-08-03