Metabolome analysis of head and neck cancer to identify the clinical markers

2012 Fiscal Year Final Research Report

• Project/Area Number: 23659795
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Kobe University
• Principal Investigator: NIBU Ken-ichi 神戸大学, 大学院・医学研究科, 教授 (20251283)
• Co-Investigator(Kenkyū-buntansha): OTSUKI Naoki 神戸大学, 大学院・医学研究科, 准教授 (40343264) ; YOSHIDA Suguru 神戸大学, 大学院・医学研究科, 准教授 (00419475) ; YONEZAWA Koichiro 神戸大学, 医学部附属病院, 医員 (20533714)
• Project Period (FY): 2011 – 2012
• Keywords: 頭頸部癌 / プロテオソーム / 腫瘍マーカー

Research Abstract

In this study, metabolome analysis of the patients with HNSCC was performed using gas chromatography mass spectrometry. Serum and tissue samples were analyzed. In serum,levels of severalmetabolites related to the glycolytic pathway were revealed to be higher in the patients with HNSCC, and the levels of severalamino acids were lower. In contrast to the sera, the levels of severalmetabolites related to the glycolytic pathway were revealed to be lower in the tumor tissues of HNSCC than the non-tumor tissues, and the levels of severalamino acids were higher. Our results demonstrate that changes in the metabolite pattern are useful for assessing the clinical characteristics of HNSCC, and will hopefully lead to the establishment of novel diagnostic tools.

Research Products

• [Journal Article] Gene silencing with siRNA targeting E6/E7 as a therapeutic intervention against head and neck cancer-containing HPV16 cell lines (2013)
  • Author(s): Adhim Z, Otsuki N, Kitamoto J, Morishita N, Kawabata M, Shirakawa T, Nibu KI
  • Journal Title: Acta Otolaryngol
• [Journal Article] an adenovirus- carrying endostatin gene, dramatically increased the tumor drug concentration of metronomic chemotherapy with low-dose cisplatin in a xenograft mouse model for head and neck squamous-cell carcinoma (2012)
  • Author(s): Adhim Z, Lin X, Huang W, Morishita N, Nakamura T, Yasui H, Otsuki N, Shigemura K, Fujisawa M, Nibu K, Shirakawa T.
  • Journal Title: Cancer Gene Ther Volume: 19 Pages: 144-152
• [Journal Article] In vitro and in vivo inhibitory effect of three Cox-2 inhibitors and epithelial-to-mesenchymal transition in human bladder cancer cell lines (2011)
  • Author(s): Adhim Z, Matsuoka T, Bito T, Shigemura K, Lee KM, Kawabata M, Fujisawa M, Nibu K, Shirakawa T
  • Journal Title: Br J Cancer Volume: 105 Pages: 393-402
• [Presentation] Serum and tissue metabolomics of head and neck cancer (2011)
  • Author(s): Yonezawa K, Otsuki N, Nibu K, et al
  • Organizer: he 11th Japan-Taiwan Conference on Otolaryngology- Head and Neck Surgery
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2011-12-25
• [Presentation] 頭頸部癌におけるメタボローム解析 (2011)
  • Author(s): 米澤宏一郎 吉田 優 丹生健一 他
  • Organizer: 第3回臨床メタボロミクス研究会
  • Place of Presentation: 神戸
  • Year and Date: 2011-10-25