2012 Fiscal Year Final Research Report
Using C. elegansas a model for studying human cancer: Establishment of C. elegansstem cell line and analysis of tumor and glycome-related genes.
| Project/Area Number |
23701060
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | University of Miyazaki (2012)
Kumamoto University (2011) |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | C. elegans / 癌 / 幹細胞 / 糖鎖 / 腫瘍 / 分化 |
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| Research Abstract |
Notch signaling is important for differentiation of germ-line stem cells (GSCs) in Caenorhabditis elegans. Upon depletion of the core components of the Notch signaling pathway, GSCs undergo abnormal mitosis and resulted in germlinetumor. In addition, other phenotypes, such as squashed vulva (Sqv), embryonic lethal (Emb) and decreased number of germ cells were also observed. According to our RNAi experiments of 145 human glycosyltransferase in C. elegans, similar phenotype was induced by knockdown of glycosaminoglycan synthases. These results strongly suggest the importance of glycosaminoglycans in the Notch signaling pathway in worms, andthe GSCs of C. eleganshave proven to be a good model for studying the mechanisms underlying stem cell differentiation and tumorigenesis.
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Research Products
(23 results)
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[Journal Article] Glioma Initiating Cells Form a Differentiation Niche Via the Induction of Extracellular Matrices and Integrin aV2013
Author(s)
Akiko Niibori-Nambu, Uichi Midorikawa, Souhei Mizuguchi, Takuichiro Hide, Minako Nagai,Yoshihiro Komohara, Megumi Nagayama, Mio Hirayama, Daiki Kobayashi, Nobuyuki Tsubota,Tatsuya Takezaki, Keishi Makino, Hideo Nakamura, Motohiro Takeya, Junichi Kuratsu, Norie Araki
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Journal Title
PLoS ONE
Volume: 8(5)
Pages: e59558
DOI
Peer Reviewed
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[Journal Article] Integrated proteomics identified novel activation of dynein IC2-GR-COX-1 signaling in NF1 disease model cells.2013
Author(s)
Hirayama M, Kobayashi D, Mizuguchi S, Morikawa T, Nagayama M, Midorikawa U, Wilson MM, Nambu AN, Yoshizawa AC, Kawano S, Araki N
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Journal Title
Molecular & Cellular Proteomics
Volume: 5(12)
Pages: 1377-1394
DOI
Peer Reviewed
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[Journal Article] Epiplakin modifies the motility of the HeLa cells and accumulates at the outer surfaces of 3-D cell clusters.2013
Author(s)
Shimada H, Nambu-Niibori A, Wilson-Morifuji M, Mizuguchi S, Araki N, Sumiyoshi H, Sato M, Mezaki Y, Senoo H, Ishikawa K, Hatano Y, Okamoto O, Fujiwara S
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Journal Title
The Journal of Dermatology
Volume: 4(40)
Pages: 249-258
DOI
Peer Reviewed
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[Journal Article] GPI-anchor Synthesis Is Indispensable for the Germline Development of the Nematode Caenorhabditis elegans.2012
Author(s)
Murata D, Nomura KH, Dejima K, Mizuguchi S, Kawasaki N, Matsuishi-Nakajima Y, Ito S, Gengyo-Ando K, Kage-Nakadai E, Mitani S, Nomura K
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Journal Title
Molecular Biology of the Cell
Volume: 6
Pages: 982-995
DOI
Peer Reviewed
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[Journal Article] Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division.2011
Author(s)
omura KH, Murata D, Hayashi Y, Dejima K, Mizuguchi S, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Hirabayashi Y, Ito M, Nomura K
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Journal Title
Glycobiology
Volume: 6
Pages: 834-848
DOI
Peer Reviewed
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[Presentation] GPI アンカー合成は線虫Caenorhabditis elegansの配偶子幹細胞ニッチの維持と生殖系列細胞発生に必須である2012
Author(s)
村田 大輔, 野村 和子, 出嶋 克史, 水口惣平, 川崎 ナナ, 中島 紫, 伊藤 さつき, 安藤 恵子, 中台 枝里子, 三谷 昌平, 松田 采子, 野村 一也
Organizer
第85回 日本生化学会大会
Place of Presentation
福岡
Year and Date
20121214-16
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[Presentation] An integrated proteomics by iPEACH, a new application, identified novel activated signal cascades in chemotherapy resistant malignant gliomas.2012
Author(s)
Norie Araki, Souhei Mizuguchi, Takashi Morikawa, Shin Kawano, Atsuko Yamaguchi, Daiki Kobayashi, Mio HIrayama, Uichi Midorikawa, Hideo Nakamura, Junichi Kuratsu
Organizer
HUPO 11th Annual World Congress
Place of Presentation
Boston, Massachusetts, USA
Year and Date
20120909-13
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[Presentation] Integrated Proteomics Identified Translationally Controlled Tumor Protein as a Biological Target for Neurofibroma and Malignant Peripheral Nerve Sheath Tumors.(口頭)2012
Author(s)
Daiki Kobayashi, Mio Hirayama, Yoshihiro Komohara, Souhei Mizuguchi, Masayo Wilson-Morifuji, Hironobu Ihn, Motohiro Takeya, Akira Kuramochi2, Norie Araki
Organizer
HUPO 11th Annual World Congress
Place of Presentation
Boston, Massachusetts, USA
Year and Date
20120909-13
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