2012 Fiscal Year Final Research Report
Development of one-step protein expression and purification systemUsing silica particles
| Project/Area Number |
23780085
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | シリカ / タンパク質発現 |
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| Research Abstract |
The aim of this study is to develop novel protein expression and purification system using silica-inducible promoter and silica-binding domain. Transcriptional analysis revealed that iron deficient caused by colloidal silica activated this promoter and ferric uptake regulator (Fur: TTHA0255) regulated this promoter. Almost the same phenomenon was seen in E. coli, and we have succeeded in the construction of silica-inducible expression vector. However, conditions for one-step expression and purification have not completed.
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