Molecular basis of hematopoietic stem and progenitor niche formation

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790533
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology
• Research Institution: Kyoto University
• Principal Investigator: OMATSU Yoshiki 京都大学, 再生医科学研究所, 助教 (80437277)
• Project Period (FY): 2011 – 2012
• Keywords: サイトカイン / 造血幹細胞ニッチ

Research Abstract

We have shown that CXC chemokine ligand (CXCL)12-abundant reticular (CAR) cells have potential to differentiate into adipocytes and osteoblasts,and play an essential role in maintenance of hematopoietic stem and progenitor cells (HSPCs) in vivo, meeting the criteria for “niche”. However, themolecular basis of the formation of cellular niches remain unclear. In this study, we found several candidate genes that play essential roles for developmentand maintenance of CAR cells, using microarrayandqRT-PCR analysis, and we generated conditional knock-out mice of these genes.

Research Products

• [Journal Article] Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration (2012)
  • Author(s): Nakamura-Ishizu A., Okuno Y., Omatsu Y., Okabe K., Morimoto J., Uede T., Nagasawa T., Suda T., Kubota Y
  • Journal Title: Blood Volume: 119(23) Pages: 5429-5437
  • DOI: doi:10.1182/blood-2011-11-393645.
  • Peer Reviewed
• [Journal Article] Control of hematopoietic stem cells by the bone marrow stromal niche: the role of reticular cells. (2011)
  • Author(s): Nagasawa T., Omatsu Y., Sugiyama T
  • Journal Title: Trends Immunol. Volume: 32(7) Pages: 315-320
  • DOI: doi:10.1016/j.it.2011.03.009.
  • Peer Reviewed
• [Journal Article] Isolation and function of mouse tissue resident vascular precursors marked by myelin protein zero (2011)
  • Author(s): Kubota Y., Takubo K., Hirashima M., Nagoshi N., Kishi K., Okuno Y., Nakamura-Ishizu A., Sano K., Murakami M., Ema M., Omatsu Y., Takahashi S., Nagasawa T., Shibuya M., Okano H., Suda T.
  • Journal Title: J. Exp. Med Volume: 208(5) Pages: 949-960
  • DOI: doi:10.1084/jem.20102187.
  • Peer Reviewed