Evaluation ofexosomes incerebrospinal fluid

2012 Fiscal Year Final Research Report

• Project/Area Number: 23790993
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Hiroshima University
• Principal Investigator: UENO Hiroki 広島大学, 病院, 病院助教 (30457238)
• Project Period (FY): 2011 – 2012
• Keywords: バイオマーカー / 脳神経疾患 / 神経科学 / RNA

Research Abstract

We analyzed particles (average diameter ~ 100 nm) in cerebrospinal fluid (CSF) by nanopore-based particle detection system and detected around billion particles per ml of CSF. Electropherograms demonstrated RNA presumably derived from exosomes in CSF with average of ~900pg/ul and analysis using miRNA array chip could identify more than 200 species of miRNAs. We also explored the possibility of IgM type antibody for superficial antigens on exosome ascross-linker that can precipitate exosomes of CSF. These results indicate that miRNAs reside in exosome of CSF are novel candidate for biomarkers of neurological disorders.

Research Products

• [Journal Article] Neuropharmacokinetic heterogeneity of mefloquine in the treatment of progressive multifocal leukoencephalopathy (2012)
  • Author(s): Ueno H, Matsumoto M
  • Journal Title: Intern Med Volume: 51(16) Pages: 2259
  • DOI: DOI:10.2169/internalmedicine.51.8079
• [Journal Article] The clinical characteristics of spinocerebellar ataxia 36: A study of 2121 Japanese ataxia patients (2012)
  • Author(s): Sugihara K, Maruyama H, Morino H, Miyamoto R, Ueno H, Matsumoto M, Kaji R, Kitaguchi H, Yukitake M, Higashi Y, Nishinaka K, Oda M, Izumi Y, Kawakami H.
  • Journal Title: Mov Disord Volume: 41 Pages: 1158-63
  • DOI: DOI:10.1002/mds.25092
• [Journal Article] 多発性巨大軟部腫瘤を認めた脳腱黄色腫症の1例 (2012)
  • Author(s): 梅本勇基,上野弘貴, 原 直之, 中村 毅, 大槻俊輔, 柳瀬哲至,山脇健盛, 松本昌泰.
  • Journal Title: 日本内科学会雑誌 Volume: 101(5) Pages: 1397-400
• [Journal Article] Akinetic Mutism Caused by HIV-associated Progressive Multifocal Leukoencephalopathy was Successfully Treated with Mefloquine: A Serial Multimodal MRI Study (2012)
  • Author(s): Naito K, Ueno H, Sekine M, Kanemitsu M, Ohshita T, Nakamura T, Yamawaki T, Matsumoto M.
  • Journal Title: Intern Med Volume: 51(2) Pages: 205-9
  • DOI: DOI:10.2169/internalmedicine.51.6253
• [Journal Article] ステロイド療法が奏功した中枢神経原発リンパ腫様肉芽腫症の 1 例 (2012)
  • Author(s): 島田さやか,上野弘貴,山崎文之,杉山一彦,安冨浩子,関根真悠,大下智彦,倉重毅志,中村 毅,西原広史,山脇健盛,栗栖 薫,松本昌泰
  • Journal Title: Brain Nerve Volume: 64(1) Pages: 85-7
• [Journal Article] 両側視床病変が遅発性に出現した悪性リンパ腫関連傍腫瘍性辺縁系脳炎の1例 (2012)
  • Author(s): 金光宗一,上野弘貴,内藤かさね,関根真悠,大下智彦,中村 毅,山脇健盛,松本昌泰
  • Journal Title: Brain Nerve Volume: 64(1) Pages: 71-7
• [Journal Article] Effect of age on the fractional anisotropy (FA) value of peripheral nerves and clinical significance of the age-corrected FA value for evaluating polyneuropathies (2012)
  • Author(s): Tanitame K, Iwakado Y, Akiyama Y, Ueno H, Ochi K, Otani K, Takasu M, Date S, Awai K
  • Journal Title: Neuroradiology Volume: 54(8) Pages: 815-21
  • DOI: DOI:10.1007/s00234-011-0981-9.
• [Journal Article] Severe brain atrophy after long-term survival seen in siblings with familial amyotrophic lateral sclerosis and a mutation in the optineurin gene: a case series. (2011)
  • Author(s): Ueno H, Kobatake K, Matsumoto M, Morino H, Maruyama H and Kawakami H.
  • Journal Title: Journal of Medical Case Reports. Volume: 5(1) Pages: 573
  • DOI: DOI:10.1186/1752-1947-5-573.