Epigenomic analysis and development of therapeutic target for malignant melanoma

2013 Fiscal Year Final Research Report

• Project/Area Number: 23791283
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Sapporo Medical University
• Principal Investigator: SATO AKIKO 札幌医科大学, 医学部, 助教 (10468093)
• Project Period (FY): 2011 – 2013
• Keywords: 悪性黒色腫 / 癌幹細胞

Research Abstract

To identify subpopulation having stem cell characteristics in melanoma, we isolated cells by fluorescence-activated sorting and implanted cells to NOD/SCID mouse and performed sphere formation assay. ABCB5 positive subpopulation in AKI melanoma cells and CD271 positive cells in WM266 cells were more tumorigenic than CD271 negative cells or ABCB5 negative cells when grafted to mice. We also analyzed slow cycling, label retaining cell. The rate of sphere formation of label retaining cells was higher than the other cells and label retaining cells showed increased expression of CD271 and ABCB5. These result show these subpopulation in melanoma cells have stem cell characteristics. We think additional analysis of epigenetic change of these cells can be used to detect new therapeutic target for melanoma.

Research Products

• [Journal Article] Aberrant methylation of microRNA-34b/c is a predictive marker of metachronous gastric cancer risk (2013)
  • Author(s): Suzuki R, Yamamoto E, Nojima M, Maruyama R, Yamano HO, Yoshikawa K, Kimura T, Harada T, Ashida M, Niinuma T, Sato A, Nosho K, Yamamoto H, Kai M, Sugai T, Imai K, Suzuki H, Shinomura Y.
  • Journal Title: J Gastroenterol Volume: (Epub ahead of print)
  • DOI: 10.1007/s00535-013-0861-7
  • Peer Reviewed
• [Journal Article] Molecular dissection of premalignant colorectal lesions reveals early onset of the CpG island methylator phenotype (2012)
  • Author(s): Yamamoto E, Suzuki H, Yamano HO, Maruyama R, Nojima M, Kamimae S, Sawada T, Ashida M, Yoshikawa K, Kimura T, Takagi R, Harada T, Suzuki R, Sato A, Kai M, Sasaki Y, Tokino T, Sugai T, Imai K, Shinomura Y, Toyota M.
  • Journal Title: Am J Pathol Volume: 181 Pages: 1847-61
  • DOI: 10.1016/j.ajpath.2012.08.007
  • Peer Reviewed
• [Presentation] DGKGによる悪性黒色腫の浸潤抑制機構の解析 (2013)
  • Author(s): 佐藤亜紀子、甲斐正広、山本英一郎、丸山玲緒、西坂尚弘、山下利春、鈴木拓
  • Organizer: 第7回日本エピジェネティクス研究会総会
  • Place of Presentation: 奈良
  • Year and Date: 2013-05-30
• [Presentation] DGK-γは黒色腫細胞の浸潤に関与する (2012)
  • Author(s): 佐藤亜紀子、甲斐正広、鈴木拓、西坂尚弘、肥田時征、米田明弘、山下利春
  • Organizer: 日本研究皮膚科学会第37回年次学術大会・総会
  • Place of Presentation: 沖縄
  • Year and Date: 2012-12-07
• [Presentation] DGK-γは黒色腫細胞の浸潤に関与する (2012)
  • Author(s): 佐藤亜紀子、甲斐正広、鈴木拓、山本英一郎、丸山玲緒、西坂尚弘、山下利春
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-20