2012 Fiscal Year Final Research Report
Relationship between lung metastases of osteosarcoma and coagulation-fibrinolytic factors
| Project/Area Number |
23890070
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | 骨肉腫 / 肺転移 / プラスミノーゲン活性化因子インヒビタ-1 (PAI-1) / ウロキナーゼ型プラスミノ-ゲン活性化因子 / 蛍光蛋白 |
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| Research Abstract |
With use of human osteosarcoma cell line “143B dual color”, in which GFP is expressing in nucleus and RFP is expressing in cytoplasm, we first developed multiple cell lines that have multiple metastatic potential. In each cell line, we evaluated expression level of uPA, PAI-1, and TF by ELIZA and PCR. The result showed strong relationship between metastatic potential and expression of uPA & PAI-1. We have now performed shRNA of uAP & PAI-1 for 143B cell line. Then we will evaluate how metastatic potential change after suppression of uPA and PAI-1 in osteosarcoma.
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Research Products
(10 results)
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[Journal Article] Imaging the efficacy of UVC irradiation on superficial brain tumors and metastasis in live mice at the subcellular level.2013
Author(s)
Momiyama M, Suetsugu A, Kimura H, Kishimoto H, Aki R, Yamada A, Sakurada H, Chishima T, Bouvet M, Endo I, Hoffman RM.
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Journal Title
J Cell Biochem
Volume: 114 (2)
Pages: 428-34
DOI
Peer Reviewed
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[Journal Article] Activity of bone morphogenetic protein-7 after treatment at various temperatures: freezing vs. pasteurization vs. allograft.2011
Author(s)
Takata M, Sugimoto N, Yamamoto N, Shirai T, Hayashi K, Nishida H, Tanzawa Y,Kimura H, Miwa S, Takeuchi A, Tsuchiya H
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Journal Title
Cryobiology.
Volume: 63(3)
Pages: 235-9
DOI
Peer Reviewed
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