Ozone-oxidized cholesterols as biomarkers for metabolic syndrome and life-style related diseases

2014 Fiscal Year Final Research Report

• Project/Area Number: 24300257
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: University of Shizuoka
• Principal Investigator: OHSHIMA Hiroshi 静岡県立大学, 食品栄養科学部, 教授 (80433209)
• Co-Investigator(Kenkyū-buntansha): MIYOSHI Noriyuki 静岡県立大学, 食品栄養科学部, 助教 (70438191) ; GODA Toshinao 静岡県立大学, 食品栄養科学部, 教授 (70195923) ; TOMONO Susumu 静岡県立大学, 食品栄養科学部, 客員研究員 (60554011) ; MIYOSHI Noriyuki 静岡県立大学, 食品栄養科学部, 客員教授 (00094342)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 健康と食生活 / オゾン酸化コレステロール / 生活習慣病

Outline of Final Research Achievements

We have newly identified fatty-acid ester forms of secosterol-A including 9-oxononanoate secosterol-A and their secosterol-B derivatives as ozone-oxidized cholesterol fatty acid esters. These ester forms of secosterols were detected by a highly sensitive and specific LC-ESI-MS/MS method at 10~20 pmol/LDL mg protein in human low density lipoproteins obtained from healthy subjects. We further improved our method to analyze comprehensively various reactive aldehydes and carbonyls present in biological samples. We are currently using the method to study roles of reactive aldehydes and carbonyls in the development of various life-style related diseases such as arteriosclerosis, neurodegenerative diseases and cancer and also validate these reactive aldehydes including secosterols and unknowns as biomarkers for oxidative stress related disorders.

Free Research Field

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