2014 Fiscal Year Final Research Report
The study for cerebellar cortical information processing using functional imaging and three-dimensional reconstruction
| Project/Area Number |
24500476
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Fusional basic brain science
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| Research Institution | The Institute of Physical and Chemical Research (2013-2014)
Saitama University (2012) |
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Principal Investigator |
MICHIKAWA TAKAYUKI 独立行政法人理化学研究所, 光量子工学研究領域, 研究員 (90282516)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | カルシウムイメージング |
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| Outline of Final Research Achievements |
Adenovirus can be used to express genetically encoded calcium indicators into specific types of neurons, such as cerebellar Purkinje cells. In vivo two-photon imaging of a large population of Purkinje cells will be useful to identify functional circuits that participate in information processing in cerebellar cortex. In this study, I have established experimental procedures and deconvolution-based data analysis methods suitable for inferring single complex spike firings of Purkinje cells from two-photon calcium imaging data acquired from living mice expressing a genetically encoded ratiometric calcium indicator, yellow cameleon 2.60.
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| Free Research Field |
神経生理学
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