Basic studies on activities, action mechanism and application of a new anti-oomycota antibiotic

2014 Fiscal Year Final Research Report

• Project/Area Number: 24510309
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Ritsumeikan University
• Principal Investigator: IMAMURA NOBUTAKA 立命館大学, 薬学部, 教授 (10160061)
• Co-Investigator(Kenkyū-buntansha): KAWASAKI Takashi 立命館大学, 薬学部, 助教 (00408733)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 生理活性物質 / 結合タンパク質 / 生合成遺伝子

Outline of Final Research Achievements

Oomycota infect plants and animals and cause severe damage in some situation. Compound 046-E produced by microorganism possess selective activity against oomycota, therefore, it seemed to become a useful preventive agent. We investigated the target molecule of 046-E and identified nucleoside diphosphate kinase B as a binding protein, however, its enzymatic activity was not inhibited by 046-E. We also studied on effects of 046-E to aquatic plankton and preventive effects against oomycota infection on fish eggs, and it was revealed that 046-E is harmless to aquatic plankton and possess potent preventive activity against oomycota infection on fish eggs at low concentration. We attempted to clone and identify a biosynthetic gene cluster for an angucycline-type antibiotics 046-E from Streptomyces sp. TK08046. Finally, we found that a gene cluster involved in 046-E biosynthesis.

Free Research Field

微生物化学