Comprehensive study of sulfurtransferase-knockout mice associated with insufficiency of hydrogen sulfide production

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590392
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Nippon Medical School
• Principal Investigator: NAGAHARA Noriyuki 日本医科大学, 医学部, 准教授 (10208043)
• Co-Investigator(Kenkyū-buntansha): ITO Takaaki 熊本大学, 大学院生命科学研究部, 教授 (70168392) ; NAGANO Masatoshi 日本医科大学, 医学部, 講師 (60271350)
• Co-Investigator(Renkei-kenkyūsha): AKIMOTO Toshio 日本医科大学, 医学部, 准教授 (30184112)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ノックアウトマウス / メルカプトピルビン酸硫黄転移酵素 / 硫化水素 / 抗酸化作用 / 不安様行動 / メルカプト乳酸システイン尿症 / セロトニン / セロトニン受容体

Outline of Final Research Achievements

We produced 3-mercaptopyruvate sulfurtransferase-knockout mice as model of an inherited disease, mercaptolactate-cysteine disulfiduria which is complicated by mental retardation. No malformation was observed, however, anxiety-like behavior was significantly increased. In the brain, dopamine level was unchanged. On the other hand, levels of serotonin and 5-hydroxyindolamine were increased. The expression of serotonin type 1A receptor was not changed. On the other hand, serotonin type 2A receptor was significantly increased. The relationships of hydrogen sulfide and/or polysulfide to the pathogenesis are investigating.

Free Research Field

病態生化学、環境医学