An analysis of cell lineage for breast cancer by gene alteration of mitochondria DNA D-loop region

2014 Fiscal Year Final Research Report

• Project/Area Number: 24590433
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Nihon University
• Principal Investigator: MASUDA Shinobu 日本大学, 医学部, 教授 (20276794)
• Co-Investigator(Renkei-kenkyūsha): ESUMI Mariko 日本大学, 医学部, 准教授 (10147019) ; TOU Shoen 日本大学, 医学部, 助教 (20326036) ; FUCHINOUE Fumi 日本大学, 医学部, 助手 (10409021) ; AMANO Sadao 日本大学, 医学部, 准教授 (80159459) ; SAKURAI Kenichi 日本大学, 医学部, 講師 (20366602)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 乳癌 / 遺伝子変異多様性 / クロナリティ / ミトコンドリアDNA D-loop領域

Outline of Final Research Achievements

(1)Gene alteration of mtDNA-DL (GAMDDL) was detected in metachronously resected ipsilateral normal breast (0.03%)and contralateral breast (0.74%).(2)Breast cancers with polysomy of centromere 17 and those with HER2 gene heterogeneity were not overlapped. (3)The protocol to visualize HER2 protein and gene simultaneously was established.(4)Primary breast cancer with heterogeneity, e.g. with and without HER2 gene amplification, and recurrent tumor without HER2 gene amplification was analyzed by GAMDDL method. There are differences of MDDL between carcinoma cells of primary tumor with and without HER2 gene amplification.

Free Research Field

人体病理学