2014 Fiscal Year Final Research Report
Isolation of cancer stem cells by the staining nucleic acid and analyses of their molecular specificities
| Project/Area Number |
24590688
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHNISHI Kazuknori 浜松医科大学, 医学部付属病院, 教授 (80252170)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 核酸 / がん幹細胞 / 蛍光染色 / 細胞分離 |
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| Outline of Final Research Achievements |
It is important to analyze chemotherapy-resistant cells after the treatment of anti-cancer drugs, because it might be useful to monitor the depth of remission and detect earlier relapse. We tried to analyze these cells alive by molecular methods after we get alive chemotherapy-resistant cells. The probes against bcr-abl fusion RNA was biotinized and then labelled fluorescences. We analyzed the fluorescence after energy transfer after insertion of the probes and incubation. The intensity of fluorescence was increased compared with bcr-abl negative cells. However it was not enough to separate them by flow cytometry. Another effort will be needed to increase the sensitivity.
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| Free Research Field |
検査腫瘍学
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