2014 Fiscal Year Final Research Report
Role of a novel LST8-binding protein on mTOR signal and glucose and lipid metabolism
| Project/Area Number |
24591315
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | University of Miyazaki (2014)
The University of Tokyo (2012-2013) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | インスリンシグナル伝達系 / mTOR |
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| Outline of Final Research Achievements |
mTOR signal is activated by overnutrition and obesity, and is strongly related with glucose and lipid metabolic disorder. The novel ProteinX was identified as a LST8, which is a common component of mTORC1 and mTORC2, by MEF-tagged pulldown assay. In this study I tried to reveal the role of ProteinX on mTOR signal. GST fusion protein of Protein X and adenovirus were constituted and the association between LST8 and ProteinX was examined by GST-pulldown assay and co-immunoprescipitation. But I could not prove the association between them. I speculate that ProteinX is the protein complex consisted with 8 isoforms, thus one of them can not bind with LST8.
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| Free Research Field |
内科学
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