Genomic alterations and molecular diversity of chimeric genes in hematologic malignancies

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591407
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TAKI Tomohiko 京都府立医科大学, 医学(系)研究科(研究院), 講師 (50322053)
• Co-Investigator(Kenkyū-buntansha): KAWAMURA Machiko 公益財団法人東京都医学研究所, 研究員 (80450592)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: がん / ゲノム / キメラ遺伝子 / 非コード遺伝子 / リボゾーム遺伝子

Outline of Final Research Achievements

Noncoding gene, PVT1 was frequently rearranged in multiple myeloma, and the PVT1-WWOX was identified in RPMI8226 cell line. We further identified another fusion transcript of PVT1, PVT1-NSMCE2, in acute myeloid leukemia (AML) having highly genomic amplification with 8q24. Novel fusion partner of PVT1, NSMCE2, also created BF104016-NSMCE2 fusion transcript in an AML cell line. On the other hand, we identified another type of fusion transcript involving ribosomal DNA. Three fusion transcripts involving RN28S1 were identified in T-cell acute lymphoblastic leukemia, Burkitt lymphoma, and multiple myeloma, respectively. Further analysis is needed to clarify the role of these novel types of fusion transcript in tumorigenesis.

Free Research Field

血液・腫瘍学、分子遺伝学