Photodynamic therapy using nanoparticle loaded with indocyanine green for experimental metastasis model of gastric cancer

2014 Fiscal Year Final Research Report

• Project/Area Number: 24591892
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: National Defense Medical College
• Principal Investigator: TSUJIMOTO Hironori 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 病院, 講師 (80554998)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 胃癌 / 腹膜播種 / センチネルリンパ節 / ナノ粒子 / ICG / 光線力学療法

Outline of Final Research Achievements

We established nano-particles loaded with indocyanine green (ICG) derivatives: ICG loaded lactosomes (ICGm) and investigated the diagnostic and therapeutic value of photodynamic therapy (PDT) using ICGm for experimental peritoneal dissemination and lymph node metastasis model of gastric cancer. Experimental peritoneal dissemination and lymph node metastasis model of human gastric cancer were established in nude mice. In vivo imaging revealed that peritoneal tumors were visualized through the abdominal wall in ICGm-treated mice, whereas only non-specific fluorescence was observed in ICG-treated mice. PDT reduced the total weight of the disseminated nodules and significantly improved weight loss and survival rate in ICGm-treated mice. In conclusion, ICGm can be used as a novel diagnostic and therapeutic nano-device in peritoneal dissemination of gastric cancer.

Free Research Field

消化器外科