2015 Fiscal Year Final Research Report
Elucidation of the establishment and carcinogenesis mechanism of in vitro pancreatic cancer model
| Project/Area Number |
24592042
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UCHIYAMA Kazuhisa 大阪医科大学, 医学部, 教授 (80232867)
HAYASHI Michihiro 大阪医科大学, 医学部, 教授 (90314179)
TANAKA Satoru 大阪医科大学, 医学部, 講師 (50595741)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | siRNA / 癌幹細胞 |
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| Outline of Final Research Achievements |
The present study's representative is independently developed, in the experimental system that induces the acinar-to-ductal transdifferentiation, but knockdown model using siRNA was a study to demonstrate the trait as a neoplasm, to confirm the acquisition of the trait it is diverted not been able to, the mechanism of anti-cancer agents acquired resistance of cancer stem cells, focused on the differentiation mechanism of the cancer cells. To confirm the expression of surface antigens of cancer stem cells (CD44, such as CD24) using a human pancreatic cancer cell line. After the confirmation of the expression, but was administered to immunodeficient mice for 3-dimensional culture, cancer stem cells that have been transplanted did not lead to growth. In the future, stable growth by changing the conditions continue to expand to allow confirmation.
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| Free Research Field |
膵臓外科
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