Identification of taxane-binding protein by utilizing magnetic beads to clarify the molecular mechanism how to develop taxane-resistance in prostate cancer

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592405
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Urology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TAKAHA Natuski 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (80294081)
• Co-Investigator(Kenkyū-buntansha): MIKI Tsuneharu 京都府立医科大学, 医学研究科, 教授 (10243239) ; KAWAUCHI Akihiro 滋賀医科大学, 医学部, 教授 (90240952) ; KAMOI Kazumi 京都府立医科大学, 医学研究科, 講師 (40295663) ; OKIHARA Koji 京都府立医科大学, 医学研究科, 准教授 (80285270) ; KIMURA Yasunori 京都府立医科大学, 医学研究科, 助教 ; UEDA Takashi 京都府立医科大学, 医学研究科, 助教 (50601598) ; IWATA Tsuyoshi 京都府立医科大学, 医学研究科, 客員講師 (00552209) ; OISHI Masakatsu 京都府立医科大学, 医学研究科, 助教 (90405316) ; UEDA Saya (ITO Saya) 京都府立医科大学, 医学研究科, 助教 (90534511)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 前立腺癌 / 抗癌剤耐性 / タキサン / ナノビーズ / ケミカルバイオロジー

Outline of Final Research Achievements

We identified tubulin, coatomer protein complex subunit alpha, and basonuclin-1 as docetaxel-binding protein overexpressed in docetaxel-resistant subcell line established from prostate cancer cell line DU145 by utilizing magnetic beads. The relative RNA expression level (docetaxel resistant/sensitive) of those molecules was about 70%, 100%, and 20%, respectively. As tubulin, which is a target molecule for docetaxel was identified, the applied method should be appropriate as purifying docetaxel-binding protein. However, the expression profile of RNA and that of protein did not coincide, which made it difficult to judge the role of the identified docetaxel-binding protein in the development of docetaxel-resistance.

Free Research Field

泌尿器腫瘍学