Regulation of drug permeability of the blood-inner ear barrier by RNA interference method

2014 Fiscal Year Final Research Report

• Project/Area Number: 24592548
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Kobe University
• Principal Investigator: TANIMOTO Hitoshi 神戸大学, 医学(系)研究科(研究院), 研究員 (50362786)
• Co-Investigator(Kenkyū-buntansha): OTSUKI Naoki 神戸大学, 医学(系)研究科(研究院), 准教授 (40343264) ; YAMASHITA Daisuke 神戸大学, 医学部附属病院, 准教授 (60306785) ; KATSUNUMA Sayaka 神戸大学, 医学部附属病院, 助教 (80457043) ; FUJITA Takeshi 神戸大学, 医学部附属病院, 助教 (90533711)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 内耳 / 血液-内耳関門 / 感音難聴 / RNA干渉

Outline of Final Research Achievements

By RNA interference method, it is possible to suppress the transcription of protein structure of the blood-inner ear barrier, and to adjust the blood-inner ear barrier barrier mechanism. For the mouse to adjust the blood-inner ear barrier barrier mechanism, intravenous drug to the systemic administration, we want to prove that the drug has reached the inner ear. It is acoustic trauma modeling gives a huge acoustic trauma, and is administered inhibit RNA interference agent through the intravenous of which is the main protein claudin-5 transcription of the blood-inner ear barrier. Then, intravenously to be administered through the brain-derived neurotrophic factor (BDNF) as a neuroprotective agent for the purpose of inner ear disorder cell regeneration. And a control mouse morphological, we went electrophysiological comparison.

Free Research Field

耳鼻咽喉科