2013 Fiscal Year Final Research Report
Cell Differentiation by DNA Quaternary Complex to Nucleus Event
| Project/Area Number |
24650281
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 遺伝子複合体 / 細胞分化誘導 / アルキルイミダゾール末端修飾PEG / DNA一分子修飾 |
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| Research Abstract |
The new concept of DNA "mono-ion complex" had been established from the DNA modification method by the interaction of the alkyl group with the DNA groove. The resulting mono-ion complex has exhibited significant higher transfection activity for tibialis muscle than clinical naked DNA and commercial in vivo transfection reagent jet PEI.
The achievement of transfection to mouse tibialis muscle cells with less mitosis suggests the possibility of exogenous gene transfection preserving their nucleus membrane. Therefore, the resulting tool to control nucleus event has been established for realizing the differentiation of cell with no mitosis.
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