Analysis of DNA adducts damage as a sensitive probe for the sick building syndrome

2015 Fiscal Year Final Research Report

• Project/Area Number: 24655143
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental chemistry
• Research Institution: Saga University
• Principal Investigator: Kondo Toshihiro 佐賀大学, 総合分析実験センター, 教務員 (20186852)
• Co-Investigator(Kenkyū-buntansha): Terato Hiroaki 佐賀大学, 総合分析実験センター, 准教授 (00243543) ; Tokuyama Yuka 佐賀大学, 総合分析実験センター, 教務員 (30398135) ; Ichiba Masayoshi 佐賀大学, 医学部, 教授 (60184628)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 核酸関連化学 / シックハウス症候群 / 質量分析

Outline of Final Research Achievements

The present study examined the methods of analysis of DNA adducts damage in carcinogenesis as a result of formaldehyde is the causative agent of sick house syndrome is induced. It was investigated separation conditions by high-performance liquid chromatography of deoxyribonucleotide triphosphates (dNTPs), which is a standard substance. Find the signal of dNTP- amino acid adduct by HPLC carried out in vitro reaction experiment of dNTP and amino acid with formaldehyde was detected signal peak, but was not confirmed by mass spectrometry. It was confirmed the separation of the four types of standard dNTPs in the high-performance liquid chromatography mass spectrometer (LC / MS).

Free Research Field

環境化学分析