Development of universal method for genome modification using zinc finger nuclease

2014 Fiscal Year Final Research Report

• Project/Area Number: 24657156
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Hiroshima University
• Principal Investigator: YAMAMOTO Takashi 広島大学, 理学(系)研究科(研究院), 教授 (90244102)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: バイオテクノロジー / ゲノム編集

Outline of Final Research Achievements

In this study, I tried to establish the method for gene modifications such as targeted gene knockout and gene knock-in in various animal species using artificial endonucleases (ZFN and TALEN). Using ZFN protein targeting sea urchin HesC gene generated by E. coli, I observed mutations in early stage of sea urchin embryo, indicating that microinjection of ZFN protein can efficiently introduce mutations in target gene compared to that of ZNF mRNA. Moreover, I established construction system for a highly active TALEN (Platinum TALEN) and demonstrated efficient targeted gene disruption using Platinum TALENs in various animal species such as roundworm (C. elegans), fruit fly (D. melanogaster), sea squirt (C. intestinalis), sea urchin (H. pulcherrimus), frog (Xenopus laevis) and newt (P. waltl)

Free Research Field

ゲノム生物学