Research on the molecular mechanisms changing taste sensitivity by internal physiological status.

2013 Fiscal Year Final Research Report

• Project/Area Number: 24658115
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: The University of Tokyo
• Principal Investigator: OKADA Shinji 東京大学, 農学生命科学研究科, 准教授 (50376563)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 味覚 / 栄養 / 食 / トランスクリプトーム

Research Abstract

Internal physiological status such as eating and nutritional status influences taste sensation. It has revealed about this phenomenon that several hormones and small molecules affect the peripheral taste receptor cells directly and modify the sensitivity to tastants. However, many parts of this mechanism remain unknown. Behavioral physiological analyses suggested the differences in taste sensitivity to one tastant between eating and non-eating animals in this study. This study also suggested the differences in gene expression profile of taste bud cells between two eating status. These results suggested that the mechanisms which modify the taste sensitivity by changing the gene expression exist in the peripheral taste receptor cells.

Research Products

• [Journal Article] Two distinct determinants of ligand specificity in T1R1/T1R3 (the umami taste receptor) (2013)
  • Author(s): Toda, Y., Nakagita, T., Hayakawa, T., Okada, S., Narukawa, M., Imai, H., Ishimaru, Y.
  • Journal Title: J. Biol. Chem. Volume: 288 Pages: 36863-36877
  • DOI: 10.1074/jbc.M113.494443
  • Peer Reviewed
• [Journal Article] Influence of a short-term iron-deficient diet on hepatic gene expression profiles in rats (2013)
  • Author(s): Kamei, A., Watanabe, Y., Kondo, K., Okada, S., Shinozaki, F., Ishijima, T., Nakai, Y., Kondo, T., Arai, S., and Abe, K.
  • Journal Title: PLoS ONE Volume: 8 Pages: e65732
  • DOI: 10.1371/journal.pone.0065732
  • Peer Reviewed
• [Journal Article] Differential Expression Analysis throughout the Weaning Period in the Mouse Cerebral Cortex (2013)
  • Author(s): Maeda, N., Kawakami, S., Ohmoto, M., Coutre, J. L., Vinyes-Pares, G., Arigoni, F., Okada, S., Abe, K., Aizawa, H., and Misaka, T.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 431 Pages: 437-443
  • DOI: 10.1016/j.bbrc.2012.12.150
  • Peer Reviewed
• [Journal Article] Transgenic labeling of higher-order neuronal circuits linked to phospholipase C-beta2-expressing taste bud cells in medaka fish (2013)
  • Author(s): Ieki, T., Okada, S., Aihara, Y., Ohmoto, M., Abe, K., Yasuoka, A., and Misaka, T.
  • Journal Title: J. Comp. Neurol. Volume: 521 Pages: 1781-1802
  • DOI: 10.1002/cne.23256
  • Peer Reviewed
• [Journal Article] Identification of a new IgE-binding epitope of peanut oleosin that cross-reacts with buckwheat (2012)
  • Author(s): Kobayashi, S., Katsuyama, S., Wagatsuma, T., Okada, S., and Tanabe, S.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 76 Pages: 1182-1188
  • DOI: 10.1271/bbb.120063
  • Peer Reviewed
• [Journal Article] 亜鉛欠乏と味覚障害・食行動異常 (2012)
  • Author(s): 岡田晋治
  • Journal Title: 生物の科学遺伝 Volume: 66 Pages: 643-648
  • URL: http://ci.nii.ac.jp/naid/40019473291
• [Journal Article] Dietary zinc status reversibly alters both the feeding behaviors of the rats and gene expression patterns in diencephalon (2012)
  • Author(s): Okada, S., Abuyama, M., Yamamoto, R., Kondo, T., Narukawa, M., and Misaka, T.
  • Journal Title: Biofactors Volume: 38 Pages: 203-218
  • DOI: 10.1002/biof.1007
  • Peer Reviewed
• [Presentation] 旨味受容体T1R1/T1R3のL-Glu活性を決定する因子の同定 (2014)
  • Author(s): 戸田安香、中北智哉、岡田晋治、成川真隆、石丸喜朗、三坂巧
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス(神奈川)
  • Year and Date: 20140327-30
• [Presentation] マウス有郭乳頭における味蕾マーカーとしてのUlex europaeus agglutinin-1の有用性 (2014)
  • Author(s): 吉本靖東、岡田晋治、山上圭吾、三坂巧
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス(神奈川)
  • Year and Date: 20140327-30
• [Presentation] メダカ味覚受容体T1R1の転写制御配列の取得 (2014)
  • Author(s): 池永直弥、吉田晃子、家木誉史、岡田晋治、三坂巧
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス(神奈川)
  • Year and Date: 20140327-30
• [Presentation] メダカにおける味覚関連遺伝子群の発現様式 (2014)
  • Author(s): 家木誉史、池永直弥、吉田晃子、岡田晋治、三坂巧
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス(神奈川)
  • Year and Date: 20140327-30
• [Presentation] 短期亜鉛欠乏におけるラットの味嗜好性変化の解析 (2013)
  • Author(s): 山本遼、岡田晋治、三坂巧
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内北キャンパス(宮城)
  • Year and Date: 20130324-28
• [Presentation] 食餌性鉄欠乏ラットにおける味嗜好性の変化 (2013)
  • Author(s): 幸田理恵、成川真隆、山本遼、岡田晋治、三坂巧
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学川内北キャンパス(宮城)
  • Year and Date: 20130324-28
• [Presentation] Taste system in fish (2012)
  • Author(s): Abe, K., Okada, S. and Misaka, T.
  • Organizer: XVI International Symposium on Olfaction and Taste (ISOT 2012)
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 20120623-27
• [Presentation] Establishment of a new cell-based assay to measure the sweetness intensities of ligands including fluorescent substances (2012)
  • Author(s): Toda, Y., Okada, S., and Misaka, T.
  • Organizer: XVI International Symposium on Olfaction and Taste (ISOT 2012)
  • Place of Presentation: Stockholm, Sweden
  • Year and Date: 20120623-27
• [Book] 新・櫻井総合食品事典 (2012)
  • Author(s): 岡田晋治, ら執筆(櫻井芳人監修、荒井綜一・倉田忠男・田島眞編集)
  • Total Pages: 1183
  • Publisher: 同文書院
• [Book] 食物と栄養学基礎シリーズ7基礎栄養学(吉田勉監修、佐藤隆一郎・加藤久典編) (2012)
  • Author(s): 岡田晋治, ら
  • Total Pages: 160
  • Publisher: 学文社
• [Remarks] 小型魚類メダカをモデルとした味の情報伝達・処理に関わる神経回路の標識
  • URL: http://www.a.u-tokyo.ac.jp/topics/2013/20130415-1.html
• [Remarks] 旨味受容体T1R1/T1R3のアミノ酸選択性を決定する分子メカニズムの同定
  • URL: http://www.a.u-tokyo.ac.jp/topics/2013/20130415-1.html
• [Remarks] 東京大学大学院農学生命科学究科ILSI Japan寄付講座「機能性食品ゲノミクス」
  • URL: http://park.itc.u-tokyo.ac.jp/ilsi/index.html