2013 Fiscal Year Final Research Report
Infection of hepatitis C virus-related non-primate hepacivirus in Japan and analysis on its replication mechanism
| Project/Area Number |
24659204
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | University of Yamanashi |
|---|
Principal Investigator |
MORIISHI Kohji 山梨大学, 医学工学総合研究部, 教授 (90260273)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YAMASHITA Atsuya 山梨大学, 医学工学総合研究部, 助教 (00334871)
KASAI Hirotake 山梨大学, 医学工学総合研究部, 助教 (20324189)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | C型肝炎ウイルス / 人獣共通感染症 / 非霊長類ヘパシウイルス / フラビウイルス |
|---|
| Research Abstract |
The objective of this study is to clarify if non-primate hepacivirus (NPHV) infects dogs or other animals in Japan. NPHV has been recently reported as a close homologue of hepatitis C virus (HCV). We prepared nose swabs from 11 dogs but did not find NPHV gene in those samples by RT-PCR. We also attempted to identify the virus from Japanese-born horses. Sera were prepared from 30 horses and were subjected to genetic analyses using RT-PCR. We identified equine hepacivirus (EHcV) in 30% preparations and determined a whole genome sequence. Non-translated regions and closed regions exhibit a similar structure to those of HCV, although 3'untranslated region of EHcV had not been reported. These results suggest that EHcV infects Japanese-born horses at a high rate in Japan compared to other countries.
|
-
[Journal Article] Inhibitory effects of caffeic Acid phenethyl ester derivatives on replication of hepatitis C virus2013
Author(s)
Shen H, Yamashita A, Nakakoshi M, Yokoe H, Sudo M, Kasai H, Tanaka T, Fujimoto Y, Ikeda M, Kato N, Sakamoto N, Shindo H, Maekawa S, Enomoto N, Tsubuki M, Moriishi K.
-
Journal Title
PLOS one
Volume: 8
Pages: e82299
DOI
Peer Reviewed
-
[Journal Article] Ca(2+) /S100 proteins regulate HCV virus NS5A-FKBP8/FKBP38 interaction and HCV virus RNA replication2013
Author(s)
Tani J, Shimamoto S, Mori K, Kato N, Moriishi K, Matsuura Y, Tokumitsu H, Tsuchiya M, Fujimoto T, Kato K, Miyoshi H, Masaki T, Kobayashi R.
-
Journal Title
Liver Int
Volume: 33
Pages: 1008-1018
DOI
Peer Reviewed
-
[Journal Article] Understanding the Biological Context of NS5A-Host Interactions in HCV Infection : A Network-Based Approach2013
Author(s)
Tripathi LP, Kambara H, Chen YA, Nishimura Y, Moriishi K, Okamoto T, Morita E, Abe T, Mori Y, Matsuura Y, Mizuguchi K.
-
Journal Title
J. Proteome Res
Volume: 12
Pages: 2537-2551
DOI
Peer Reviewed
-
-
-
[Journal Article] Inhibition of both protease and helicase activities of hepatitis C virus NS3 by an ethyl acetate extract of marine sponge Amphimedon sp2012
Author(s)
Fujimoto Y, Salam KA, Furuta A, Matsuda Y, Fujita O, Tani H, Ikeda M, Kato N, Sakamoto N, Maekawa S, Enomoto N, de Voogd NJ, Nakakoshi M, Tsubuki M, Sekiguchi Y, Tsuneda S, Akimitsu N, Noda N, Yamashita A, Tanaka J, Moriishi K.
-
Journal Title
PLoS One
Volume: 7
Pages: e48635
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
[Presentation]2013
Author(s)
Tanaka T, Kasai H, Yamashita A, Moriishi K.
Organizer
20^<th> International Symposium on Hepatitis C virus and related viruses
Place of Presentation
Melbourne, Australia
Year and Date
20131006-10
-
-
-
-
[Presentation] FKBP6 plays an important role in HCV replication through binding to HCV NS5A2012
Author(s)
Kasai H., Kawakami, K., Yamashita, A., Ikeda, M., Kato, N., Enomoto, N., Matsuura, Y., Kusunoki, M., Moriishi, K.
Organizer
19^<th> International Symposium on Hepatitis C virus and related viruses
Place of Presentation
Venice, Italy
Year and Date
20121005-09
