Development of methods to inhibit tumorigenesis after transplantation of differentiated iPS cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24659575
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: National Defense Medical College
• Principal Investigator: MATSUMURA Kouji 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 医学教育部共同利用研究施設, 講師 (30272610)
• Co-Investigator(Renkei-kenkyūsha): NAKAI Kanji 福岡大学, 医学部, 講師 (20420838) ; HAYASHI Katsuki 防衛医科大学校病院, 講師 (10532517) ; ISHIHARA Miya 防衛医科大学校, 医学教育部, 教授 (30505342) ; IHSIHARA Masayuki 防衛医科大学校, 防衛医学研究センター (10508500) ; ARAI Hitoaki 防衛医科大学校, 医学教育部, 助教 (50534864)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: iPS細胞 / 腫瘍 / 放射線照射 / 5FU / 温熱 / 分化誘導

Outline of Final Research Achievements

In transplant medical treatment of the iPS cells, most etiology of tumor genesis after the transplant depends on undifferentiated iPS cells remaining in induced differentiation tissues.
Since the irradiation showed high sensitivity to undifferentiated cells, the induced differentiation cells of human iPS cells and mouse iPS cells were irradiated. As results, tumor genesis was significantly reduced when it was transplanted into mouse. The hyperthermia showed sensitivity at 43.5 degrees Celsius to undifferentiated iPS cells. However, in transplant experiment, the significant difference was not observed. By flow cytometer, anticancer agent (5FU) was useful for a removal of the undifferentiated iPS cells. Therefore, we advance transplant experiment. For graft cells of the human iPS cells, these tumor inhibition methods can reduce a ratio of residual undifferentiated iPS cells and these methods will help safe transplant treatment.

Free Research Field

再生医療学