2013 Fiscal Year Final Research Report
Role of autophagy in immunoescape of solid tumor
| Project/Area Number |
24659599
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KITAYAMA JOJI 東京大学, 医学部附属病院, 准教授 (20251308)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMASHITA Hiroharu 東京大学, 医学部付属病院, 助教 (50599397)
YAMAGUCHI Hironori 東京大学, 医学部付属病院, 助教 (20376445)
KAWAI Kazushige 東京大学, 医学部付属病院, 助教 (80571942)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 低酸素 / 低栄養 / autophagy |
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| Research Abstract |
We established a mouse model to investigate the local immune reaction in regeonal lymphnodes. Using this model, we found that Chloroquine, an autophagy inhibitor, significantly potentiated the antitumor activity of Temsirolimus in vitro and in vivo. The combination therapy triggered enhanced apoptosis, which corresponded to an increased Bax/Bcl-2 ratio. In human, we also found that a minor subset of intraperitoneal free cells with CD90(+)CD45(-) phenotype vigorously grow in culture with mesothelial-like morphology. Interestingly, these cultured cells were similar to mesenchymal stem cells (MSC), and were designated as intraperitoneal MSC (pMSC). The growth of pMSC is strongly inhibited by hypoxia or hypoglycemia possibly through autophagy.
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Research Products
(7 results)
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[Journal Article] Temsirolimus and chloroquine cooperatively exhibit a potent antitumor effect against colorectal cancer cells2014
Author(s)
Kaneko M, Nozawa H, Hiyoshi M, Tada N, Murono K, Nirei T, Emoto S, Kishikawa J, Iida Y, Sunami E, Tsuno NH, Kitayama J, Takahashi K, Watanabe T
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Journal Title
J Cancer Res Clin Oncol
Volume: 140(5)
Pages: 769-81
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