2013 Fiscal Year Final Research Report

Analysis of N-glycans associated with diabetic retinopathy

Research Project

Project/Area Number	24659754
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Single-year Grants
Research Field	Ophthalmology
Research Institution	Hokkaido University
Principal Investigator	ISHIDA Susumu 北海道大学, 医学(系)研究科(研究院), 教授 (10245558)
Co-Investigator(Kenkyū-buntansha)	NISHIMURA Shin-ichiro 北海道大学, 先端生命科学研究科, 教授 (00183898) AMANO Maho 北海道大学, 創成研究機構, 特任助教 (80365808)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	糖尿病網膜症 / 糖鎖 / グライコブロッティング
Research Abstract	Glycans are biopolymers bearing biological information and regarded as the third major class of cellular macromolecules, following nucleic acids and proteins. Glycosylation is an important process of post-translational modification that changes the functions of proteins. In this study, we analyzed the profile of N-glycans in human vitreous fluid obtained from patients with non-proliferative vitreoretinal diseases such as epiretinal membrane and macular hole (n=20). The concentration of N-glycans in vitreous samples was significantly lower compared with those in plasma samples. In both samples, predominant N-glycan was common and the structure is [(Hex)2 (HexNAc)2 (NeuAc)2 + (Man)3(GlcNAc)2].