2013 Fiscal Year Final Research Report
Development of the iPS cell-based diagnostic technology for dental treatments
| Project/Area Number |
24659858
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
EGUSA Hiroshi 大阪大学, 歯学研究科(研究院), 助教 (30379078)
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| Co-Investigator(Kenkyū-buntansha) |
YATANI Hirofumi 大阪大学, 大学院歯学研究科, 教授 (80174530)
NAKANO Tamaki 大阪大学, 大学院歯学研究科, 助教 (40379079)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | iPS細胞 / 骨芽細胞 / 個体差 |
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| Research Abstract |
Induced pluripotent stem (iPS) cells, which can be generated individual patients, have potential to represent the patient-specific cell responses in vitro. In this study, we attempted to generate integration-free iPS cells from patients' gingiva using episomal plasmid vectors, and successfully generated iPS cell clones. We next evaluated optimal osteogenic induction of iPS cells using mouse gingiva-derived iPS cells, demonstrating that iPS cells can be guided to differentiate into mature osteoblasts with hydroxyapatite formation. These results suggest that the firm method to guide integration-free iPS cells to differentiate into osteoblasts may lead to a new technology for detecting individual differences in the clinical outcome of bone regeneration.
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