Study on new metabolisms in plant vacuole by integration of the post-genome analysis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24710235
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied genomics
• Research Institution: Kobe University
• Principal Investigator: OHNISHI Miwa 神戸大学, 理学(系)研究科(研究院), 助教 (10437501)
• Research Collaborator: YAMAMOTO Kotaro 神戸大学 ; ANEGAWA Aya 神戸大学 ; MIMURA Tetsuro 神戸大学 ; FUKAKI Hidehiro 神戸大学 ; ISHIZAKI Kimitsune 神戸大学 ; SHICHIJO Chizuko 神戸大学 ; MURAKAMI Akio 神戸大学 ; MASUJIMA Tsutomu 理化学研究所 ; MIZUNO Hajime 理化学研究所 ; TAKAHASHI Katsutoshi 産業技術総合研究所 ; YAMAZAKI Mami 千葉大学
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 液胞 / ニチニチソウ / 二次代謝 / ポストゲノム解析

Outline of Final Research Achievements

Vacuole is an important organelle in the plant cell. It occupies about 80% of the plant cell volume. It is known that a variety of substances are accumulated in the vacuole. In this study, we focused on the biosynthesis of alkaloids that are accumulated in the vacuole of Catharanthus roseus, this is one of famous medical plants. From previous studies, alkaloids have been shown to be synthesized through various tissues and organelles. We analyzed the localization of alkaloids and their intermediates by using new technology. There are some different results from previous reports. These results suggest the existence of novel metabolic functions in the vacuole of Catharanthus roseus. In the future, we would like to apply the present new knowledge to the development of efficient alkaloid biosynthesis system.

Free Research Field

植物生理学