Development of high-precision quantitative techniques of sequence-specific nucleic acid molecule

2014 Fiscal Year Final Research Report

• Project/Area Number: 24750079
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: FUJII Shin-ichiro 独立行政法人産業技術総合研究所, 物質計測標準研究部門, 主任研究員 (10415739)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 核酸 / 塩基配列 / 定量分析 / 質量分析 / 分離分析

Outline of Final Research Achievements

A mass spectrometry technique for the direct quantification of a nucleic acid molecule of the polymer had saved the nucleotide sequence was performed in this research subject.
We realized the direct quantification of a high-molecular nucleic acid using combining techniques of separation such as high-performance liquid chromatography (HPLC) or capillary electrophoresis and inductively coupled plasma mass spectrometry (ICP-MS). The phosphorus in the molecule of nucleic acid was measured by these analytical methods. Especially, in the method using size exclusion chromatography (SEC) hyphenated with ICP-MS, it was possible to quantify the RNA molecules of 500 to 1000 bases. These results showed the effectiveness and practicality of this approach.

Free Research Field

分析化学