Function and regulation of D-amino acid in eukaryote

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780098
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: ITO Tomokazu 名古屋大学, 生命農学研究科, 助教 (90584970)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: D-アミノ酸 / D-セリン / D-アスパラギン酸 / D-セリンデヒドラターゼ

Outline of Final Research Achievements

We developed a simple and rapid D-serine enzymatic assay kit applicable to determine D-serine content in human urine. We studied the reaction mechanisms of two D-serine metabolic enzymes, serine racemase and Zn2+-dependent D-serine dehydratase. We characterized the enzymatic propeｒties of putative D-aspartate biosynthetic enzyme in mammals, Got1l1, and analyzed the effects of knock-out of the Got1l1 on the D-aspartate production. The obtained results showed that the Got1l1 is not the major D-aspartate synthetic enzyme in mammals.

Free Research Field

生化学　酵素学