2013 Fiscal Year Final Research Report
Development of the vaccine delivery method against fish diseases using virus-like particle
| Project/Area Number |
24780198
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General fisheries
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| Research Institution | Fisheries Research Agency |
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Principal Investigator |
TAKANO Tomokazu 独立行政法人水産総合研究センター, 増養殖研究所, 研究員 (40533998)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ウイルス様粒子 / ワクチン / ベータノダウイルス |
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| Research Abstract |
Virus-like particles (VLP) may be utilized as vaccine vector of oral and/or immersion vaccination of fish. Hence, generation of VLP from the recombinant coat protein of viral nervous necrosis (VNN) was assessed in this study. Recombinant coat protein was generated by Escherichia coli BL21 harboring the coat protein gene. The recombinant coat protein was effectively purified by a sucrose density-gradient centrifugation method. However, irregular-shaped VLPs were observed in the sample by electron microscopy.
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