Molecular analysis of immune privilege in pluripotent stem cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790276
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Kyoto University
• Principal Investigator: OKITA Keisuke 京都大学, iPS細胞研究所, 講師 (90512434)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 再生医学 / 多能性幹細胞

Research Abstract

Induced pluripotent stem (iPS) cells showed slight upregulation of HLA expression by the stimulation with IFNgamma, but it was ten fold lower than that in fibroblasts. On the other hand, we have recently developed an efficient method to make iPS cells from human peripheral blood. This study enabled us to establish iPS cells from Macaca fascicularis. Transplantation of differentiated cells from monkey iPS cells can be a useful model to examine human immune response after transplantation.

Research Products

• [Journal Article] Direct Comparison of Autologous and Allogeneic Transplantation of iPSC-Derived Neural Cells in the Brain of a Nonhuman Primate (2013)
  • Author(s): Morizane A, Doi D, Kikuchi T, Okita K, Hotta A, Kawasaki T, Hayashi T, Onoe H, Shiina T, Yamanaka S, and Takahashi J
  • Journal Title: Stem Cell Reports Volume: 1 Pages: 283-292
  • DOI: 10.1016/j.stemcr.2013.08.007
  • Peer Reviewed
• [Journal Article] An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells Stem Cells (2013)
  • Author(s): Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, and Yamanaka S
  • Volume: 31
  • Pages: 458-466
  • DOI: 10.1002/stem.1293
  • Peer Reviewed
• [Journal Article] Donor-dependent variations in hepatic differentiation from human-induced pluripotent stem cells (2012)
  • Author(s): Kajiwara M, Aoi T, Okita K, Takahashi R, Inoue H, Takayama N, Endo H, Eto K, Toguchida J, Uemoto S, and Yamanaka S
  • Journal Title: Proc Natl Acad Sci U S A Volume: 109 Pages: 12538-12543
  • DOI: 10.1073/pnas.1209979109
  • Peer Reviewed
• [Presentation] iPS細胞の臨床応用を目指して日本薬学会関東支部 (2013)
  • Author(s): 沖田圭介
  • Organizer: 第38回学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2013-12-22
  • Invited
• [Presentation] iPS細胞研究の最前線から岩形態が変形しディッシュから剥離していき、安定した培養と実験系の構築は困難であった (2013)
  • Author(s): 沖田圭介
  • Organizer: 国市科学センター市民科学講座
  • Place of Presentation: 山口
  • Year and Date: 2013-03-16
  • Invited
• [Presentation] iPS細胞を、医療へ (2013)
  • Author(s): 沖田圭介
  • Organizer: 大阪大学付属病院未来医療センター10周年・未来医療開発部開設記念シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2013-01-13
  • Invited
• [Presentation] iPS細胞の臨床応用を目指して (2012)
  • Author(s): 沖田圭介
  • Organizer: 北海道大学第一回IGM特別セミナー
  • Place of Presentation: 北海道
  • Year and Date: 2012-12-20
  • Invited
• [Presentation] Re-characterization of iPS cells (2012)
  • Author(s): Keisuke Okita
  • Organizer: Netherlands - Japan Cross Debate Workshop on RM and Stem cells
  • Place of Presentation: 神奈川
  • Year and Date: 2012-06-17
  • Invited
• [Presentation] Immunogenic potential of mouse pluripotent stem cells (2012)
  • Author(s): Keisuke Okita, Yasuko Matsumura, Yoshiko Sato, and Shinya Yamanaka
  • Organizer: ISSCR 10th annual Meeting
  • Place of Presentation: 神奈川
  • Year and Date: 2012-06-15
• [Book] 細胞工学 (2012)
  • Author(s): 島本廉, 沖田圭介
  • Total Pages: 308-313
  • Publisher: 学研メディカル秀潤社
• [Book] 実験医学増刊 (2012)
  • Author(s): 山川達也, 沖田圭介
  • Total Pages: 101-106
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/okita/