2013 Fiscal Year Final Research Report
In-vivo Imaging of Brain Abnormalities Induced by Prenatal Exposure to Radiation in Rodents
| Project/Area Number |
24791299
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Osaka University |
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Principal Investigator |
SAITO Shigeyoshi 大阪大学, 医学(系)研究科(研究院), 助教 (40583068)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 胎生期被ばく / 放射線 / 生体イメージング / マイクロCT / 小動物MRI / 組織染色 |
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| Research Abstract |
We assessed brain abnormalities in mice and rats exposed prenatally to radiation (X-rays) using magnetic resonance imaging (MRI), Micro-CT and histological experiments. These animals were exposed to different radiation doses (0.5, 1.0, and 1.5 Gy). Brain abnormalities were assessed in neonatal male mice and rats. In vivo/Ex vivo T2-weighted imaging and diffusion tensor imaging (DTI) were performed using 11.7 T MRI. Decreased brain volume, increased ventricle volume, and thinner cortices were observed in irradiated rats and mice using MRI. However, no abnormalities in the cortical 6-layered structure were observed in radiation-exposed mice and rats. T2W and DTI and histological experiments are useful tools for assessing cortical and hippocampal abnormalities after prenatal exposure to radiation in mice and rats.
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