Objective diagnostic procedure of the microinvasion of the oral mucosal malignancies

2013 Fiscal Year Final Research Report

• Project/Area Number: 24792186
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Niigata University
• Principal Investigator: KOBAYASHI Takanori 新潟大学, 医歯学系, 助教 (00464016)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 口腔癌 / 初期浸潤 / 上皮内癌 / 異型上皮 / 術中迅速診断 / 再発

Research Abstract

It is difficult to determine the early microinvasion of oral squamous cell carcinoma histopathologically. Therefore we focused the production and deposition of the extracellular matrix (ECM), and investigated whether there was characteristic ECM deposition in the oral invasive cancer.
As a result, the thickening of basement membrane was characteristic in the carcinoma in-situ (CIS), and excessive deposition of basement membrane molecules at the thickening zone were confirmed immunohistochemically. Furthermore, ECM molecules, especially perlecan, were deposited in the epithelial layers of the epithelial dysplasia and CIS, whereas their deposition switched into the cancer stroma at the invasive stage.
In conclusion, the switching of area of ECM deposition could be regarded as an indicator of early invasion of oral cancer.

Research Products

• [Journal Article] ITGA3 and ITGB4 expression biomarkers estimate the risks of locoregional and hematogenous dissemination of oral squamous cell carcinoma (2013)
  • Author(s): Nagata M, Noman AA, Suzuki K, Kurita H, Ohnishi M, Ohyama T, Kitamura N, Kobayashi T, Uematsu K, Takahashi K, Kodama N, Kawase T, Hoshina H, Ikeda N, Shingaki S, Takagi R
  • Journal Title: BMC Cancer Volume: 13 Pages: 410
  • Peer Reviewed
• [Journal Article] Inflammatory histopathogenesis of nasopalatine duct cyst : a clinicopathological study of 41 cases (2013)
  • Author(s): Tsuneki M, Maruyama S, Yamazaki M, Abe T, Adeola HA, Cheng J, Nishiyama H, Hayashi T, Kobayashi T, Takagi R, Funayama A, Saito C, Saku T
  • Journal Title: Oral disease Volume: 19 Pages: 415-424
  • Peer Reviewed
• [Journal Article] Keratin 10-positive orthokeratotic dysplasia : a new leucoplakia-type precancerous entity of the oral mucosa (2012)
  • Author(s): Kobayashi T, Maruyama S, Abe T, Cheng J, Takagi R, Saito C, Saku T
  • Journal Title: Histopathology Volume: 61 Pages: 910-920
  • Peer Reviewed
• [Journal Article] Short telomeres in an oral precancerous lesion : Q-FISH analysis of leukoplakia (2012)
  • Author(s): Aida J, Kobayashi T, Saku T , Yamaguchi M, Shimomura N, Nakamura K, Ishikawa N, Maruyama S, Cheng J, Poon SS, Sawabe M, Arai T, Takubo K
  • Journal Title: J Oral Pathology and Medicine Volume: 41(5) Pages: 372-378
  • Peer Reviewed
• [Journal Article] Clinicopathological distinction of two categories of oral squamous cell carcinoma of the tongue : de novo vs. sequential type (2012)
  • Author(s): Saito M, Kobayashi T, Takagi R, Saku T
  • Journal Title: Oral Medicine and Pathology Volume: 81 Pages: 81-88
  • Peer Reviewed
• [Journal Article] Ghost cell odontogenic carcinoma arising in the background of a benign calcifying cystic odontogenic tumor of the mandible : Report of a case and review of the literature (2012)
  • Author(s): Arashiyama T, Kodama Y, Kobayashi T, Hoshina H, Takagi R, Hayashi T, Cheng J, Saku T
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Volume: 114(3) Pages: 35-40
  • Peer Reviewed
• [Journal Article] 頭頸部癌の切除不能頸部リンパ節転移巣に対する温熱化学放射線療法, 治療成績および予後について (2012)
  • Author(s): 星名秀行, 永田昌毅, 高木律男, 藤田 一, 安島久雄, 児玉泰光, 池田順行, 齋藤正直, 小林孝憲, 嵐山貴徳, 小山貴寛, 小玉直樹, 勝見祐二, 小川 信, 山田一穂, 魚島勝美
  • Journal Title: 新潟歯学会誌 Volume: 42(1) Pages: 27-36
  • Peer Reviewed
• [Presentation] 口腔粘膜扁平上皮癌および悪性境界病変の再発に関する臨床病理学的検討 (2013)
  • Author(s): 御代田 駿, 小林孝憲, 宮島 久, 小林正治, 高木律男, 丸山 智, 朔 敬
  • Organizer: 第23回日本口腔内科学会・第26回口腔診断学会合同学術大会
  • Place of Presentation: 東京
  • Year and Date: 20130913-14
• [Presentation] 粘膜下口蓋裂に関する臨床的検討-診断と治療について- (2013)
  • Author(s): 小林孝憲, 大湊 麗, 児玉泰光, 小山貴寛, 永田昌毅, 飯田明彦, 小野和宏, 高木律男
  • Organizer: 第37回日本口蓋裂学会総会・学術総会
  • Place of Presentation: 佐賀市
  • Year and Date: 20130530-31
• [Presentation] 口腔扁平上皮癌とその境界病変における術中迅速病理診断の意義 : 局所再発に関する臨床病理学的検討 (2013)
  • Author(s): 御代田 駿, 小林孝憲, 宮島 久, 永田昌毅, 星名秀行, 小林正治, 高木律男, 丸山 智, 朔 敬
  • Organizer: 平成25年度新潟歯学会第二回例会
  • Place of Presentation: 新潟市
  • Year and Date: 2013-11-09
• [Presentation] 口腔扁平上皮癌とその境界病変における術中迅速病理診断の意義 : 再発に関する臨床病理学的検討 (2013)
  • Author(s): 御代田 駿, 小林孝憲, 宮島 久, 小林正治, 高木律男, 丸山 智, 朔 敬
  • Organizer: 第73回新潟癌治療研究会
  • Place of Presentation: 新潟市
  • Year and Date: 2013-07-27
• [Presentation] 下歯槽神経障害を伴う放射線性骨髄炎を呈した頭頸部癌の 2 例 (2013)
  • Author(s): 村山正晃, 小林孝憲, 児玉泰光, 池田順行, 永田昌毅, 高木律男
  • Organizer: 第17回口腔顔面神経機能学会
  • Place of Presentation: 福岡市
  • Year and Date: 2013-03-02
• [Presentation] インテグリン遺伝子発現定量による扁平上皮癌のリンパ行性及び血行性転移のリスク診断 (2012)
  • Author(s): 永田昌毅, 栗田 浩, 星名秀行, 大西 眞, 北村信隆, 小林孝憲, 上松晃也, 新垣晋, 齊藤 力, 高木律男
  • Organizer: 第57回日本口腔外科学会総会・学術大会
  • Place of Presentation: 横浜市
  • Year and Date: 20121019-21
• [Presentation] 舌癌化学療法中に生じた抗利尿ホルモン不適合症候群(SIADH)の 1 例 (2012)
  • Author(s): 小林孝憲, 安楽純子, 齊藤直朗, 飯田明彦, 大西眞, 高木律男
  • Organizer: 第38回(社)日本口腔外科学会北日本地方会
  • Place of Presentation: 札幌市
  • Year and Date: 20120623-24
• [Presentation] 上唇と咬筋内に発生した静脈奇形の 1 例 (2012)
  • Author(s): 小林孝憲, 安島久雄, 小山貴寛, 齋藤正直, 村山正晃, 高木律男
  • Organizer: 日本形成外科学会関東支部第88回新潟地方会
  • Place of Presentation: 新潟市
  • Year and Date: 2012-11-30