Biological nitrification inhibition (BNI) activity in wild-wheat (Leymus racemosus), and its chemical and genetical characterizations

2015 Fiscal Year Final Research Report

• Project/Area Number: 25292039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Japan International Research Center for Agricultural Sciences
• Principal Investigator: Subbarao Guntur 国立研究開発法人国際農林水産業研究センター, 生産環境・畜産領域, 主任研究員 (00442723)
• Co-Investigator(Kenkyū-buntansha): NAKAHARA Kazuhiko 国立研究開発法人国際農林水産業研究センター, 生物資源・利用領域, プロジェクトリーダー (90241778) ; ANDO Yasuo 国立研究開発法人国際農林水産業研究センター, 生産環境・畜産領域, プロジェクトリーダー (80353548) ; TSUJIMOTO Hisashi 鳥取大学, 乾燥地研究センター, 教授 (50183075)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 生物的硝化抑制 / BNI / コムギ / 遠縁野生種 / 気候変動 / 肥料利用効率 / 亜酸化窒素

Outline of Final Research Achievements

The high-BNI capacity of wild wheat, Leymus racemosus (LR) is mostly controlled by Lr#N chromosome, expressed in Chinese Spring. Further, the short-arm of the Lr#N chromosome controls BNI-capacity. BNI-trait from Lr#N expressed in cultivated wheat only when translocated on 3B, but not on 7B wheat-chromosome. Three shortened-short-arm-Lr#N translocations were developed on to 3B chromosome and were evaluated for BNI-capacity. One of the translocations in CS genetic background, nearly doubled the BNI-activity of root systems and also has a positive effect on wheat growth. The BNI activity purification reached an intermediate stage with several active peaks were identified and in the process of purification. Out of seventy primers designed and tested, only twenty primers amplified PCR products from L. racemosus. Further work is needed to design additional primers that can work for N chromosome in CS background.

Free Research Field

土壌・肥料学