The basic research of preclinical simulation system for chemotherapy using "individual Cancer Xenografts"

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430098
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Central Institute for Experimental Animals
• Principal Investigator: KAWAI KENJI 公益財団法人実験動物中央研究所, その他部局等, 研究員 (30414064)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Masato 東海大学, 医学部, 教授 (00164335)
• Co-Investigator(Renkei-kenkyūsha): MIYAGI Yohei 神奈川県立がんセンター, 臨床研究所, 部長 (00254194)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 疾患モデル / 個別化医療 / NOGマウス

Outline of Final Research Achievements

We established rapid and effective protocols for making individual Cancer Xenograft (iCX). In the iCXs, morphological characteristics, gene expression profiles, and genetic alteration patterns were all well preserved. In colorectal cancer iCXs, Collagen gel droplet embedded culture-drug sensitivity tests (CD-DST) were performed. The CD-DST results between original and iCX specimens were generally correlated (R2=0.01-0.89). The result of CD-DST using iCX was compatible with clinical effects of anti-cancer drug. Zinc finger protein 185 (ZNF185) is one of proteins selected from proteome analysis utilizing cancer xenograft models. We also analyzed the association between ZNF185 and the clinicopathological characteristics of colon cancer. ZNF185 expression was found to be an independent indicator of liver metastasis and prognosis in patients with colon cancer. Our iCXs with clinical information are a promising advance that may accelerate the development of anticancer therapies.

Free Research Field

腫瘍病理学