The morphology of small intestine and the restorative effect on drug induced colitis in proglucagon derived peptides knockout mice

2016 Fiscal Year Final Research Report

• Project/Area Number: 25460949
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya University
• Principal Investigator: Ando Takafumi 名古屋大学, 医学系研究科, 招へい教員 (80378041)
• Research Collaborator: Hayashi Yoshitaka 名古屋大学, 環境医学研究所, 准教授
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: GLP-2 / DSS腸炎

Outline of Final Research Achievements

We elucidated the morphology of small intestine and the restoration on colitis with deficiency of proglucagon derived peptides (PGDPs) containing GLP-2. Mice deficient with PGDPs were used. Eight-week-old male mice of PGDPs-/- and PGDPs+/+ were sacrificed to check the morphology of the small intestine. To elucidate the effect of the deletion of PGDPs on colitis, 12-week-old PGDPs -/- and PGDPs+/+ male mice were exposed to 2.5% dextran sulfate sodium (DSS) for 6 days. Severity of colitis was assessed daily by body weight loss and disease activity index. Histological examinations were performed on day 7 and 21.
There were no deformity and no differences in the villous height and crypt depth in PGDPs-/- mice. In DSS colitis, the decline and amelioration of body weight and disease activity index were not different between PGDP-/- and PGDP+/+.PGDPs deletion did not showed the morphological deformity of small intestine and differences in colitis.

Free Research Field

消化器内科