The mechanism of regulation for cell polarity in heart targeting ischemic heart disease treatment

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461119
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: Nakano Atsushi 国立研究開発法人国立循環器病研究センター, 研究開発基盤センター, 室長 (90648106)
• Co-Investigator(Kenkyū-buntansha): KITAKAZE Masafumi 国立循環器病研究センター, 研究開発基盤センター, 部長 (20294069)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 循環器・高血圧 / 細胞極性 / 分子生物学

Outline of Final Research Achievements

In cell biology, AMP activated protein kinase (AMPK) is known to be a key protein kinase to regulate and maintain cell migration and polarity by phosphorylating cytoplasmic linker protein 170 (CLIP-170), a downstream target of AMPK. However, the function in vivo is not understood. In this study, we examined whether AMPK-CLIP-170 axis play a crucial role for cell migration and polarity at early development stage of heart. By using a line of zebrafish (hspGEF3A) that express target genes specifically at 1 atrial 1 ventricle zebrafish heart, we expressed mutant CLIP-170 gene resistant to phosphorylation by AMPK in heart, and found that the fish showed opposite direction of heart looping. These results suggest that AMPK-CLIP-170 axis play a crucial role for establishment of cell migration and polarity in heart.

Free Research Field

循環器内科学