Basic analysis of clinical significance of novel vasculitis biomarkers identified by a peptidomic approach

2015 Fiscal Year Final Research Report

• Project/Area Number: 25461489
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Collagenous pathology/Allergology
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Ozaki Shoichi 聖マリアンナ医科大学, 医学部, 教授 (00231233)
• Co-Investigator(Kenkyū-buntansha): KUROKAWA Manae 聖マリアンナ医科大学, 医学部, 准教授 (90301598)
• Co-Investigator(Renkei-kenkyūsha): NAGAFUCHI Hiroko 聖マリアンナ医科大学, 医学部, 講師 (80278001)
• Research Collaborator: OHMOTO Yasukazu
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ANCA関連血管炎 / ペプチドミクス / アポリポタンパク

Outline of Final Research Achievements

We established two quantitative assay systems of serum AC13 levels. One method is a competitive ELISA system with serum samples which have been applied to a centrifugal ultra- filter 10K devices in order to eliminate serum ApoA-I that cross-react with AC13. The other method is a quantitative mass spectrometry using stable isotope-labeled AC13 as an internal control. A representative standard curve showed R2=0.9802, suggesting the usefulness of this quantitative method.
To investigate the function of AC13, we analyzed the protein profiles of human micro vascular endothelial cells (hMVEC) cultured in the presence or absence of AC13. AC13 appeared to augment the TNF-mediated inflammation and ERK1/2-induced cell proliferation and adhesion, resulting in the exacerbation of vasculitis. We also demonstrated that biotinated AC13 bound to hMVEC, suggesting the presence of AC13-bindng protein or receptor on the cell surface.

Free Research Field

リウマチ、膠原病、自己免疫学