Chorionic gonadotropin beta modulates epithelial-mesenchymal transition in human colorectal carcinoma metastasis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462044
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Hokkaido University
• Principal Investigator: Homma Shigenori 北海道大学, 大学病院, 助教 (30533674)
• Co-Investigator(Kenkyū-buntansha): NISHIHSRA HIROSHI 北海道大学, 大学院医学研究科, 特任准教授 (50322805) ; TAKAHASHI NORIHIKO 北海道大学, 北海道大学病院, 准教授 (30399894) ; KAMACHI HIROFUMI 北海道大学, 北海道大学病院, 講師 (60374237)
• Co-Investigator(Renkei-kenkyūsha): TAKETOMI AKINOBU 北海道大学, 大学院医学研究科, 教授 (70363364) ; KAWAMURA HIDEKI 北海道大学, 大学院医学研究科, 特任研究講師 (70645960)
• Research Collaborator: KAWAMATA FUTOSHI 北海道大学, 北海道大学病院, 医員
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 大腸癌 / EMT / 浸潤・転移 / hCG β

Outline of Final Research Achievements

The aim of this study was to examine the biological role of hCGβ associated with EMT and its clinical significance in colorectal cancer(CRC).
Immunohistochemically, hCGβ was positively identified in 13 of 80 tumors (16.3 %),and was correlated with histological grade (p = 0.031), lymph node metastasis (p = 0.021) and lymphatic invasion (p = 0.011) and unfavorable patients outcome (p = 0.021) compared to the group of hCGβ negative expression. Stably overexpressing hCGβ demonstrated significantly increased invasion (p < 0.05), and microarray and subsequent PCR analysis identified upregulation of Snail 2 and Twist 1 genes associtaed with hCGβ overexpression. Furthermore, Stably overexpressing hCGβ demonstrated significantly increased tumor growth in mouse peritoneal metastasis model (p < 0.005). HCGβ plays an important role in mediating EMT and metastatic behavior in CRC.

Free Research Field

消化器外科