2015 Fiscal Year Final Research Report
microRNA aberration in gliomas with IDH mutation and metastasis
| Project/Area Number |
25462258
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOHMURA Eiji 神戸大学, 医学(系)研究科(研究院), 教授 (30225388)
TANAKA Kazuhiro 神戸大学, 医学(系)研究科(研究院), 研究員 (70467661)
MIZUKAWA Katsu 神戸大学, 医学部附属病院, 助教 (80403260)
NISHIHARA Masamitsu 神戸大学, 医学(系)研究科(研究院), 研究員 (20452493)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | glioma / microRNA / IDH mutation / metastatic |
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| Outline of Final Research Achievements |
To generate glioma cells expressing isocitrate dehydrogenase-1 (IDH1) mutant protein, we cultured glioma cells from the glioma tissues that have IDH1 mutant. But, glioma cells expressing IDH1 mutant protein could not grow. When plasmides IDH1 mutant were transfected in glioma cells, glioma cell lines that stably express IDH1 mutant protein could not grow. In glioma cells that transiently express IDH1 mutant, 2HG was significantly elevated and glutamate was significantly reduced in glioma cells. Then, we analyzed microRNA expression in disseminated or metastatic glioma. In disseminated/metastatic gliomas, miR-7、miR-29b、miR-34a、miR-101、miR-124、miR-128a、miR-137、mR-218 were significantly down-regulated compared with non-metastatic gliomas. When these microRNAs' mimics were transfected in glioma cells, glioma cell migration, proliferation, and invasion were decreased. Therefore, these microRNAs could be predictive biomarker for disseminated/metastatic glioma.
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| Free Research Field |
脳腫瘍
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