Establishment of a novel method for retinal cell differentiation using pediatric eye ball and small compound

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462767
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Nishina Sachiko 国立研究開発法人国立成育医療研究センター, 感覚器・形態外科部・眼科, 医員 (40237954)
• Co-Investigator(Renkei-kenkyūsha): AZUMA Noriyuki 国立成育医療研究センター, 感覚器・形態・外科部眼科, 医長 (10159395) ; NISHINA Hiroshi 東京医科歯科大学, 難治疾患研究所, 教授 (60212122)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 網膜神経節細胞 / 神経細胞 / iPS細胞 / ES細胞

Outline of Final Research Achievements

In this study, we tried to establish to generate neuronal cells including self-induced retinal ganglion cells (RGCs). Results: 1) After development of the optic vesicle from the induced stem cell embryoid body in three-dimensional culture, conversion to two-dimensional culture, achieved by supplementation with BDNF, resulted in differentiation of RGCs; 2) We utilised a mouse embryonic stem cell differentiation system and a library of well-characterised drugs to identify these metabolic factors. We found that statins, which inhibit the mevalonate metabolic pathway, suppressed primitive streak formation in vitro and in vivo. These results may contribute to both basic and clinical science, including embryology, neurology, pathognomy, and treatment of various optic nerve diseases that threaten vision.

Free Research Field

眼科学