Evaluation of intracanal biota using metagenomics - a new strategy for endodontic treatment

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462945
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tohoku University
• Principal Investigator: YAMAKI KEIKO 東北大学, 歯学研究科(研究院), 助教 (90182419)
• Co-Investigator(Kenkyū-buntansha): SATO TAKUICHI 東北大学, 大学院歯学研究科, 講師 (10303132) ; MAYANAGI GEN 東北大学, 大学院歯学研究科, 助教 (10451600) ; SHIMAUCHI HIDETOSHI 東北大学, 大学院歯学研究科, 名誉教授 (70187425)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 歯内治療学 / 口腔細菌 / 根尖性歯周炎 / PCR法 / 16S rRNA / 嫌気性菌 / メタゲノム解析

Outline of Final Research Achievements

Intracanal microflora associated with apical periodontitis was investigated. Under informed consent of patients, dentin samples were collected and analyzed using two rapid microbial cell counters, anaerobic culture, and PCR targeting 16S rRNA genes. Bacterial species found from endodontically suffering canals showed a great diversity in both quality and quantity. Mechanical enlargement of the root canal combined with chemical disinfection could effectively reduce the pre-dominantly anaerobic pathogen. Propionibacterium sp. and Olsenella sp. were frequently recovered in contrast to previous reports. Evaluating the difference in bacterial profile between oral cavity and root canal through metagenomics could lead to a new strategy for diagnosis and treatment of apical periodontitis.

Free Research Field

医歯薬学