Cryopreservation of rat MSCs by use of a programmed freezer with magnetic field.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25463179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Kaku Masato 広島大学, 大学病院, 講師 (10325194)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨髄間葉系幹細胞 / 凍結保存 / 磁場 / 細胞移植 / 骨再生

Outline of Final Research Achievements

Survival and proliferation rates of MSCs were significantly higher in CAS freezer than in the non-magnetic freezer. Alizarin positive reaction, large amount of calcium quantification, and greater alkaline phosphatase activity were shown in both the non-cryopreserved and CAS groups after osteogenic differentiation. Moreover, Oil Red O staining positive reaction and high amount of PPARγ and FABP4 mRNAs were shown in both the non-cryopreserved and CAS groups after adipogenic differentiation.
Animal studies indicate that MSCs survival and proliferation rates were significantly higher in the CAS group than in the Direct group. In the Control and CAS groups, a large amount of new bone formation and a suture-like gap was identified 24 weeks after transplantation, whereas only a small amount of new bone formation was observed in the Direct group.

Free Research Field

再生医療